Key features and details
- Rabbit polyclonal to SF3A3
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SF3A3 antibody
See all SF3A3 primary antibodies
DescriptionRabbit polyclonal to SF3A3
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human SF3A3 aa 75-125. The exact sequence is proprietary. (NP_006793.1).
Database link: Q12874
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab224814 was affinity purified using an epitope specific to SF3A3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab224814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 59 kDa.|
RelevanceSF3A3 is subunit 3 of the splicing factor SF3A. Splicing factor 3a is a heterotrimer consisting of subunits 1, 2 and 3, and is necessary for the conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. SF3A interacts with the pre-mRNA branch site early during spliceosome formation.
Cellular localizationNucleus speckle.
- SF3A3 antibody
- Pre mRNA splicing factor SF3a (60kD) antibody
- Pre mRNA splicing factor SF3a antibody
All lanes : Anti-SF3A3 antibody (ab224814) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 59 kDa
Exposure time: 30 seconds
SF3A3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab224814 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224814 at 0.4 µg/ml.
Lane 1: ab224814 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224814 has not yet been referenced specifically in any publications.