Overview

  • Product name

  • Description

    Rabbit polyclonal to SF3A3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human SF3A3 aa 75-125. The exact sequence is proprietary. (NP_006793.1).
    Database link: Q12874

  • Positive control

    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab224814 was affinity purified using an epitope specific to SF3A3 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab224814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 59 kDa.

Target

  • Relevance

    SF3A3 is subunit 3 of the splicing factor SF3A. Splicing factor 3a is a heterotrimer consisting of subunits 1, 2 and 3, and is necessary for the conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. SF3A interacts with the pre-mRNA branch site early during spliceosome formation.
  • Cellular localization

    Nucleus speckle.
  • Database links

  • Alternative names

    • SF3A3 antibody
    • Pre mRNA splicing factor SF3a (60kD) antibody
    • Pre mRNA splicing factor SF3a antibody
    • PRP 9 antibody
    • PRP9 antibody
    • PRPF 9 antibody
    • PRPF9 antibody
    • SAP 61 antibody
    • SAP61 antibody
    • SF3a60 antibody
    • Spliceosome associated protein 61 antibody
    • Splicing factor 3a subunit 3 60kDa antibody
    • Splicing factor 3a subunit 3 antibody
    see all

Images

  • All lanes : Anti-SF3A3 antibody (ab224814) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 59 kDa


    Exposure time: 30 seconds
  • SF3A3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab224814 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224814 at 0.4 µg/ml.

    Lane 1: ab224814 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 10 seconds.

References

ab224814 has not yet been referenced specifically in any publications.

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