Recombinant Anti-SF3A3 antibody [EPR10020] (ab156873)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10020] to SF3A3
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
Overview
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Product name
Anti-SF3A3 antibody [EPR10020]
See all SF3A3 primary antibodies -
Description
Rabbit monoclonal [EPR10020] to SF3A3 -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC/IF HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human SF3A3 (Q12874).
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Positive control
- Recombinant Human SF3A3 protein (ab132617) can be used as a positive control in WB. Human fetal liver, HeLa, Raji and A431 lysates. Human lung carcinoma tissue and HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10020 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC/IF |
Human
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IHC-P |
Human
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WB |
Human
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All applications |
Mouse
Rat
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Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
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Notes |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
WB
1/1000 - 1/10000. Predicted molecular weight: 59 kDa. |
Target
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Relevance
SF3A3 is subunit 3 of the splicing factor SF3A. Splicing factor 3a is a heterotrimer consisting of subunits 1, 2 and 3, and is necessary for the conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. SF3A interacts with the pre-mRNA branch site early during spliceosome formation. -
Cellular localization
Nucleus speckle. -
Database links
- Entrez Gene: 10946 Human
- Entrez Gene: 75062 Mouse
- Entrez Gene: 313583 Rat
- Omim: 605596 Human
- SwissProt: Q12874 Human
- SwissProt: Q9D554 Mouse
- Unigene: 77897 Human
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Alternative names
- SF3A3 antibody
- Pre mRNA splicing factor SF3a (60kD) antibody
- Pre mRNA splicing factor SF3a antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)
ab156873 showing +ve staining in Human normal brain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)
ab156873 showing +ve staining in Human normal kidney.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)
ab156873 showing +ve staining in Human normal colon.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)
ab156873 showing +ve staining in Human cervical carcinoma.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)
ab156873 showing +ve staining in Human breast carcinoma.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-SF3A3 antibody [EPR10020] (ab156873) at 1/1000 dilution
Lane 1 : Human fetal liver lysates
Lane 2 : HeLa cell lysates
Lane 3 : Raji cell lysates
Lane 4 : A431 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 59 kDa -
Immunofluorescent analysis of Hela cells labeling SF3A3 with ab156873 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded lung carcinoma tissue labelling SF3A3 with ab156873 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References (3)
ab156873 has been referenced in 3 publications.
- Zheng YZ et al. PHF5A Epigenetically Inhibits Apoptosis to Promote Breast Cancer Progression. Cancer Res 78:3190-3206 (2018). PubMed: 29700004
- Wan Y et al. Splicing function of mitotic regulators links R-loop-mediated DNA damage to tumor cell killing. J Cell Biol 209:235-46 (2015). WB, IP . PubMed: 25918225
- Katsogiannou M et al. The functional landscape of Hsp27 reveals new cellular processes such as DNA repair and alternative splicing and proposes novel anticancer targets. Mol Cell Proteomics 13:3585-601 (2014). WB . PubMed: 25277244