Recombinant
RabMAb

Recombinant Anti-SF3A3 antibody [EPR10020] (ab156873)

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Overview

  • Product name

    Anti-SF3A3 antibody [EPR10020]
    See all SF3A3 primary antibodies

  • Description

    Rabbit monoclonal [EPR10020] to SF3A3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human SF3A3 (Q12874).

  • Positive control

    • Recombinant Human SF3A3 protein (ab132617) can be used as a positive control in WB. Human fetal liver, HeLa, Raji and A431 lysates. Human lung carcinoma tissue and HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR10020

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Relevance

      SF3A3 is subunit 3 of the splicing factor SF3A. Splicing factor 3a is a heterotrimer consisting of subunits 1, 2 and 3, and is necessary for the conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. SF3A interacts with the pre-mRNA branch site early during spliceosome formation.

    • Cellular localization

      Nucleus speckle.

    • Database links

      • Alternative names

        • SF3A3 antibody
        • Pre mRNA splicing factor SF3a (60kD) antibody
        • Pre mRNA splicing factor SF3a antibody
        • PRP 9 antibody
        • PRP9 antibody
        • PRPF 9 antibody
        • PRPF9 antibody
        • SAP 61 antibody
        • SAP61 antibody
        • SF3a60 antibody
        • Spliceosome associated protein 61 antibody
        • Splicing factor 3a subunit 3 60kDa antibody
        • Splicing factor 3a subunit 3 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)

        ab156873 showing +ve staining in Human normal brain.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)

        ab156873 showing +ve staining in Human normal kidney.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)

        ab156873 showing +ve staining in Human normal colon.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)

        ab156873 showing +ve staining in Human cervical carcinoma.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)

        ab156873 showing +ve staining in Human breast carcinoma.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Western blot - Anti-SF3A3 antibody [EPR10020] (ab156873)
        Western blot - Anti-SF3A3 antibody [EPR10020] (ab156873)
        All lanes : Anti-SF3A3 antibody [EPR10020] (ab156873) at 1/1000 dilution

        Lane 1 : Human fetal liver lysates
        Lane 2 : HeLa cell lysates
        Lane 3 : Raji cell lysates
        Lane 4 : A431 cell lysates

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 59 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-SF3A3 antibody [EPR10020] (ab156873)
        Immunocytochemistry/ Immunofluorescence - Anti-SF3A3 antibody [EPR10020] (ab156873)
        Immunofluorescent analysis of Hela cells labeling SF3A3 with ab156873 at 1/100 dilution.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded lung carcinoma tissue labelling SF3A3 with ab156873 at 1/100.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      References

      This product has been referenced in:

      • Wan Y  et al. Splicing function of mitotic regulators links R-loop-mediated DNA damage to tumor cell killing. J Cell Biol 209:235-46 (2015). WB, IP . Read more (PubMed: 25918225) »
      • Katsogiannou M  et al. The functional landscape of Hsp27 reveals new cellular processes such as DNA repair and alternative splicing and proposes novel anticancer targets. Mol Cell Proteomics 13:3585-601 (2014). WB . Read more (PubMed: 25277244) »
      See all 2 Publications for this product

      Publishing research using ab156873? Please let us know so that we can cite the reference in this datasheet.

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