Anti-SF3A3 antibody [EPR10020] (ab156873)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-SF3A3 antibody [EPR10020]
See all SF3A3 primary antibodies -
DescriptionRabbit monoclonal [EPR10020] to SF3A3
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human SF3A3 (Q12874).
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Positive control
- Recombinant Human SF3A3 protein (ab132617) can be used as a positive control in WB. Human fetal liver, HeLa, Raji and A431 lysates. Human lung carcinoma tissue and HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20ºC.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR10020
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab156873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/250. | |
| ICC/IF | 1/100 - 1/250. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 59 kDa. |
Target
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RelevanceSF3A3 is subunit 3 of the splicing factor SF3A. Splicing factor 3a is a heterotrimer consisting of subunits 1, 2 and 3, and is necessary for the conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. SF3A interacts with the pre-mRNA branch site early during spliceosome formation.
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Cellular localizationNucleus speckle.
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Database links
- Entrez Gene: 10946 Human
- Entrez Gene: 75062 Mouse
- Entrez Gene: 313583 Rat
- Omim: 605596 Human
- SwissProt: Q12874 Human
- SwissProt: Q9D554 Mouse
- Unigene: 77897 Human
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Alternative names
- SF3A3 antibody
- Pre mRNA splicing factor SF3a (60kD) antibody
- Pre mRNA splicing factor SF3a antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)](https://www.abcam.com/ps/products/156/ab156873/Images/ab156873-8-YCAR10020-SF3A3-brain.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)ab156873 showing +ve staining in Human normal brain. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)](https://www.abcam.com/ps/products/156/ab156873/Images/ab156873-7-YCAR10020-SF3A3-kidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)ab156873 showing +ve staining in Human normal kidney. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)](https://www.abcam.com/ps/products/156/ab156873/Images/ab156873-6-YCAR10020-SF3A3-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)ab156873 showing +ve staining in Human normal colon. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)](https://www.abcam.com/ps/products/156/ab156873/Images/ab156873-5-YCAR10020-SF3A3-cervix-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)ab156873 showing +ve staining in Human cervical carcinoma. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)](https://www.abcam.com/ps/products/156/ab156873/Images/ab156873-4-YCAR10020-SF3A3-breast-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)ab156873 showing +ve staining in Human breast carcinoma. -
![Western blot - Anti-SF3A3 antibody [EPR10020] (ab156873)](https://www.abcam.com/ps/datasheet/images/156/ab156873/SF3A3-Primary-antibodies-ab156873-2.jpg)
Western blot - Anti-SF3A3 antibody [EPR10020] (ab156873)All lanes : Anti-SF3A3 antibody [EPR10020] (ab156873) at 1/1000 dilution
Lane 1 : Human fetal liver lysates
Lane 2 : HeLa cell lysates
Lane 3 : Raji cell lysates
Lane 4 : A431 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 59 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SF3A3 antibody [EPR10020] (ab156873)](https://www.abcam.com/ps/datasheet/images/156/ab156873/SF3A3-Primary-antibodies-ab156873-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SF3A3 antibody [EPR10020] (ab156873)Immunofluorescent analysis of Hela cells labeling SF3A3 with ab156873 at 1/100 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)](https://www.abcam.com/ps/datasheet/images/156/ab156873/SF3A3-Primary-antibodies-ab156873-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] (ab156873)Immunohistochemical analysis of formalin-fixed, paraffin-embedded lung carcinoma tissue labelling SF3A3 with ab156873 at 1/100.
Datasheets and documents
Protocols
References
This product has been referenced in:
- Wan Y et al. Splicing function of mitotic regulators links R-loop-mediated DNA damage to tumor cell killing. J Cell Biol 209:235-46 (2015). WB, IP . Read more (PubMed: 25918225) »
- Katsogiannou M et al. The functional landscape of Hsp27 reveals new cellular processes such as DNA repair and alternative splicing and proposes novel anticancer targets. Mol Cell Proteomics 13:3585-601 (2014). WB . Read more (PubMed: 25277244) »
