Key features and details
- Mouse monoclonal [4G8] to SF3a66 (HRP)
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Product nameAnti-SF3a66 antibody [4G8] (HRP)
See all SF3a66 primary antibodies
DescriptionMouse monoclonal [4G8] to SF3a66 (HRP)
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Monkey
Full length native protein (purified) corresponding to Rat SF3a66. Nuclear protein isolated from rat liver nuclei.
EpitopeC-terminal, amino acids 212-464.
- IHC-P: FFPE human skin tissue sections.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab201747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionSubunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.
Sequence similaritiesBelongs to the SF3A2 family.
Contains 1 matrin-type zinc finger.
- Information by UniProt
- pre-mRNA splicing factor SF3A, subunit 2 antibody
- PRP11 antibody
- PRPF11 antibody
IHC image of SF3a66 staining in a section of formalin-fixed paraffin-embedded human normal skin*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab201747, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
ab201747 has not yet been referenced specifically in any publications.