Overview

  • Product name

    Anti-SF3a66 antibody [4G8] (HRP)
    See all SF3a66 primary antibodies
  • Description

    Mouse monoclonal [4G8] to SF3a66 (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Monkey
  • Immunogen

    Full length native protein (purified) corresponding to Rat SF3a66. Nuclear protein isolated from rat liver nuclei.

  • Epitope

    C-terminal, amino acids 212-464.
  • Positive control

    • IHC-P: FFPE human skin tissue sections.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    4G8
  • Myeloma

    Sp2/0
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab201747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.
  • Sequence similarities

    Belongs to the SF3A2 family.
    Contains 1 matrin-type zinc finger.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • pre-mRNA splicing factor SF3A, subunit 2 antibody
    • PRP11 antibody
    • PRPF11 antibody
    • SAP 62 antibody
    • SAP62 antibody
    • Sf3a2 antibody
    • SF3A2_HUMAN antibody
    • SF3a66 antibody
    • spliceosome associated protein 62 antibody
    • Spliceosome-associated protein 62 antibody
    • Splicing factor 3A subunit 2 antibody
    • splicing factor 3a, subunit 2, 66kDa antibody
    see all

Images

  • IHC image of SF3a66 staining in a section of formalin-fixed paraffin-embedded human normal skin*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab201747, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

References

ab201747 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab201747.
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