For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    sf3b1-antibody-epr11986-ab172634.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Splicing
Share by email
RecombinantRabMAb

Recombinant Anti-SF3B1 antibody [EPR11986] (ab172634)

  • Datasheet
Reviews (1) Submit a question References (6)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)
  • Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] (ab172634)
  • Flow Cytometry - Anti-SF3B1 antibody [EPR11986] (ab172634)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)
  • Anti-SF3B1 antibody [EPR11986] (ab172634)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11986] to SF3B1
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-SF3B1 antibody [EPR11986]
    See all SF3B1 primary antibodies
  • Description

    Rabbit monoclonal [EPR11986] to SF3B1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SF3B1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O75533

  • Positive control

    • WB: HeLa, Jurkat, K562 and Ramos cell lysates. IHC-P: Human colon and glioma tissues. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11986
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing

Associated products

  • Alternative Versions

    • HRP Anti-SF3B1 antibody [EPR11986] (ab202926)
    • Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (ab240170)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Ramos whole cell lysate (ab3955)
    • Jurkat whole cell lysate (ab7899)
    • K562 whole cell lysate (ab7911)

Applications

Our Abpromise guarantee covers the use of ab172634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 146 kDa.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/2000.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance

      SF3B1 is subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures.
    • Cellular localization

      Nucleus speckle. Note=During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.
    • Database links

      • Entrez Gene: 23451 Human
      • Entrez Gene: 81898 Mouse
      • Entrez Gene: 84486 Rat
      • Omim: 605590 Human
      • SwissProt: O75533 Human
      • SwissProt: Q99NB9 Mouse
      • Unigene: 632554 Human
      • Unigene: 279736 Mouse
      • Unigene: 198843 Rat
      see all
    • Alternative names

      • Hsh 155 antibody
      • MDS antibody
      • Pre mRNA processing 10 antibody
      • Pre mRNA splicing factor SF3b 155 kDa subunit antibody
      • PRP 10 antibody
      • PRP10 antibody
      • PRPF 10 antibody
      • PRPF10 antibody
      • SAP 155 antibody
      • SAP155 antibody
      • SF3B 1 antibody
      • SF3b155 antibody
      • Spliceosome associated protein 155 antibody
      • Splicing factor 3b subunit 1 155kDa antibody
      • Splicing factor 3B subunit 1 antibody
      see all

    Images

    • Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634)
      All lanes : Anti-SF3B1 antibody [EPR11986] (ab172634) at 1/1000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : Jurkat lysate
      Lane 3 : K562 lysate
      Lane 4 : Ramos lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 146 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)

      Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human colon tissue labeling SF3B1 with ab172634 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] (ab172634)

      Immunofluorescence analysis of HeLa cells labeling SF3B1 with ab172634 at 1/250 dilution.

    • Flow Cytometry - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Flow Cytometry - Anti-SF3B1 antibody [EPR11986] (ab172634)

      Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling SF3B1 (red) with ab172634 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)

      Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human glioma tissue labeling SF3B1 with ab172634 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-SF3B1 antibody [EPR11986] (ab172634)
      Anti-SF3B1 antibody [EPR11986] (ab172634)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (6)

    Publishing research using ab172634? Please let us know so that we can cite the reference in this datasheet.

    ab172634 has been referenced in 6 publications.

    • Vázquez-Borrego MC  et al. Splicing Machinery is Dysregulated in Pituitary Neuroendocrine Tumors and is Associated with Aggressiveness Features. Cancers (Basel) 11:N/A (2019). PubMed: 31561558
    • Wu X  et al. Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell 172:423-438.e25 (2018). PubMed: 29249360
    • Kyei GB  et al. Splicing Factor 3B Subunit 1 Interacts with HIV Tat and Plays a Role in Viral Transcription and Reactivation from Latency. MBio 9:N/A (2018). PubMed: 30401776
    • Murthy T  et al. Cyclin-dependent kinase 1 (CDK1) and CDK2 have opposing roles in regulating interactions of splicing factor 3B1 with chromatin. J Biol Chem 293:10220-10234 (2018). PubMed: 29764937
    • Marques F  et al. Altered mRNA Splicing, Chondrocyte Gene Expression and Abnormal Skeletal Development due to SF3B4 Mutations in Rodriguez Acrofacial Dysostosis. PLoS Genet 12:e1006307 (2016). WB ; Human . PubMed: 27622494
    • Mirtschink P  et al. HIF-driven SF3B1 induces KHK-C to enforce fructolysis and heart disease. Nature 522:444-9 (2015). WB . PubMed: 26083752

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    ChIP abreview for Anti-SF3B1 antibody [EPR11986]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - whole cell (Jurkat cells)
    Negative control
    Epsilon globin gene
    Specification
    Jurkat cells
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 40 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 30min 1.5mM EGS 10 min 1% HCHO
    Positive control
    PCNA gene
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 21 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.