Recombinant
RabMAb

Recombinant Anti-SF3B1 antibody [EPR11986] (ab172634)

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Overview

  • Product name

    Anti-SF3B1 antibody [EPR11986]
    See all SF3B1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11986] to SF3B1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human SF3B1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O75533

  • Positive control

    • WB: HeLa, Jurkat, K562 and Ramos cell lysates. IHC-P: Human colon and glioma tissues. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11986

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 146 kDa.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/2000.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Relevance

      SF3B1 is subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures.

    • Cellular localization

      Nucleus speckle. Note=During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.

    • Database links

      see all

    • Alternative names

      • Hsh 155 antibody
      • MDS antibody
      • Pre mRNA processing 10 antibody
      • Pre mRNA splicing factor SF3b 155 kDa subunit antibody
      • PRP 10 antibody
      • PRP10 antibody
      • PRPF 10 antibody
      • PRPF10 antibody
      • SAP 155 antibody
      • SAP155 antibody
      • SF3B 1 antibody
      • SF3b155 antibody
      • Spliceosome associated protein 155 antibody
      • Splicing factor 3b subunit 1 155kDa antibody
      • Splicing factor 3B subunit 1 antibody
      see all

    Images

    • Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634)
      All lanes : Anti-SF3B1 antibody [EPR11986] (ab172634) at 1/1000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : Jurkat lysate
      Lane 3 : K562 lysate
      Lane 4 : Ramos lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 146 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)

      Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human colon tissue labeling SF3B1 with ab172634 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] (ab172634)

      Immunofluorescence analysis of HeLa cells labeling SF3B1 with ab172634 at 1/250 dilution.

    • Flow Cytometry - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Flow Cytometry - Anti-SF3B1 antibody [EPR11986] (ab172634)

      Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling SF3B1 (red) with ab172634 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)

      Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human glioma tissue labeling SF3B1 with ab172634 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Kyei GB  et al. Splicing Factor 3B Subunit 1 Interacts with HIV Tat and Plays a Role in Viral Transcription and Reactivation from Latency. MBio 9:N/A (2018). Read more (PubMed: 30401776) »
    • Marques F  et al. Altered mRNA Splicing, Chondrocyte Gene Expression and Abnormal Skeletal Development due to SF3B4 Mutations in Rodriguez Acrofacial Dysostosis. PLoS Genet 12:e1006307 (2016). WB ; Human . Read more (PubMed: 27622494) »
    See all 3 Publications for this product

    Publishing research using ab172634? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    ChIP abreview for Anti-SF3B1 antibody [EPR11986]

     Poor
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - whole cell (Jurkat cells)
    Negative control
    Epsilon globin gene
    Specification
    Jurkat cells
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 40 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 30min 1.5mM EGS 10 min 1% HCHO
    Positive control
    PCNA gene
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 21 2015

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