Recombinant
RabMAb

Recombinant Anti-SF3B1 antibody [EPR11986] (ab172634)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11986] to SF3B1
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SF3B1 antibody [EPR11986]
    See all SF3B1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11986] to SF3B1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human SF3B1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O75533

  • Positive control

    • WB: HeLa, Jurkat, K562 and Ramos cell lysates. IHC-P: Human colon and glioma tissues. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11986

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 146 kDa.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/2000.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Relevance

      SF3B1 is subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures.

    • Cellular localization

      Nucleus speckle. Note=During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.

    • Database links

      see all

    • Alternative names

      • Hsh 155 antibody
      • MDS antibody
      • Pre mRNA processing 10 antibody
      • Pre mRNA splicing factor SF3b 155 kDa subunit antibody
      • PRP 10 antibody
      • PRP10 antibody
      • PRPF 10 antibody
      • PRPF10 antibody
      • SAP 155 antibody
      • SAP155 antibody
      • SF3B 1 antibody
      • SF3b155 antibody
      • Spliceosome associated protein 155 antibody
      • Splicing factor 3b subunit 1 155kDa antibody
      • Splicing factor 3B subunit 1 antibody
      see all

    Images

    • Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634)
      All lanes : Anti-SF3B1 antibody [EPR11986] (ab172634) at 1/1000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : Jurkat lysate
      Lane 3 : K562 lysate
      Lane 4 : Ramos lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 146 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)

      Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human colon tissue labeling SF3B1 with ab172634 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] (ab172634)

      Immunofluorescence analysis of HeLa cells labeling SF3B1 with ab172634 at 1/250 dilution.

    • Flow Cytometry - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Flow Cytometry - Anti-SF3B1 antibody [EPR11986] (ab172634)

      Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling SF3B1 (red) with ab172634 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)

      Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human glioma tissue labeling SF3B1 with ab172634 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References (5)

    Publishing research using ab172634? Please let us know so that we can cite the reference in this datasheet.

    ab172634 has been referenced in 5 publications.

    • Vázquez-Borrego MC  et al. Splicing Machinery is Dysregulated in Pituitary Neuroendocrine Tumors and is Associated with Aggressiveness Features. Cancers (Basel) 11:N/A (2019). PubMed: 31561558
    • Kyei GB  et al. Splicing Factor 3B Subunit 1 Interacts with HIV Tat and Plays a Role in Viral Transcription and Reactivation from Latency. MBio 9:N/A (2018). PubMed: 30401776
    • Murthy T  et al. Cyclin-dependent kinase 1 (CDK1) and CDK2 have opposing roles in regulating interactions of splicing factor 3B1 with chromatin. J Biol Chem 293:10220-10234 (2018). PubMed: 29764937
    • Marques F  et al. Altered mRNA Splicing, Chondrocyte Gene Expression and Abnormal Skeletal Development due to SF3B4 Mutations in Rodriguez Acrofacial Dysostosis. PLoS Genet 12:e1006307 (2016). WB ; Human . PubMed: 27622494
    • Mirtschink P  et al. HIF-driven SF3B1 induces KHK-C to enforce fructolysis and heart disease. Nature 522:444-9 (2015). WB . PubMed: 26083752

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    ChIP abreview for Anti-SF3B1 antibody [EPR11986]

     Poor
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - whole cell (Jurkat cells)
    Negative control
    Epsilon globin gene
    Specification
    Jurkat cells
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 40 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 30min 1.5mM EGS 10 min 1% HCHO
    Positive control
    PCNA gene
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 21 2015

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