RecombinantRabMAb

Recombinant Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (ab240170)

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Flow Cytometry - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (ab240170)
  • Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (ab240170)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (ab240170)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (ab240170)
  • Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (ab240170)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11986] to SF3B1 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SF3B1 antibody [EPR11986] - BSA and Azide free
    See all SF3B1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11986] to SF3B1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human SF3B1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O75533

  • General notes

    Ab240170 is the carrier-free version of ab172634. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240170 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11986

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab240170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 146 kDa.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Relevance

      SF3B1 is subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures.

    • Cellular localization

      Nucleus speckle. Note=During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.

    • Database links

      see all

    • Alternative names

      • Hsh 155 antibody
      • MDS antibody
      • Pre mRNA processing 10 antibody
      • Pre mRNA splicing factor SF3b 155 kDa subunit antibody
      • PRP 10 antibody
      • PRP10 antibody
      • PRPF 10 antibody
      • PRPF10 antibody
      • SAP 155 antibody
      • SAP155 antibody
      • SF3B 1 antibody
      • SF3b155 antibody
      • Spliceosome associated protein 155 antibody
      • Splicing factor 3b subunit 1 155kDa antibody
      • Splicing factor 3B subunit 1 antibody
      see all

    Images

    • Flow Cytometry - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (ab240170)
      Flow Cytometry - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (ab240170)

      Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling SF3B1 (red) with ab172634 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172634).

    • Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (ab240170)
      Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (ab240170)

      Immunofluorescence analysis of HeLa cells labeling SF3B1 with ab172634 at 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172634).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (ab240170)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (ab240170)

      Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human glioma tissue labeling SF3B1 with ab172634 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172634).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (ab240170)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (ab240170)

      Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human colon tissue labeling SF3B1 with ab172634 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172634).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (ab240170)
      Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (ab240170)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab240170? Please let us know so that we can cite the reference in this datasheet.

    ab240170 has not yet been referenced specifically in any publications.

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