Product nameAnti-SF3B1 antibody [EPR11987(B)]
See all SF3B1 primary antibodies
DescriptionRabbit monoclonal [EPR11987(B)] to SF3B1
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human SF3B1 (Cysteine residue). The exact sequence is proprietary.
Database link: O75533
- HeLa, K562 and Ramos cell lysates; Human brain tissue and colonic carcinoma tissue; HeLa cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab170854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 146 kDa.|
|ICC/IF||1/100 - 1/250.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
RelevanceSF3B1 is subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures.
Cellular localizationNucleus speckle. Note=During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.
- Hsh 155 antibody
- MDS antibody
- Pre mRNA processing 10 antibody
Immunofluorescent analysis of HeLa cells labeling SF3B1 with ab170854 at 1/100 dilution.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colonic carcinoma tissue labeling SF3B1 with ab170854 at 1/100 dilution.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling SF3B1 with ab170854 at 1/100 dilution.
All lanes : Anti-SF3B1 antibody [EPR11987(B)] (ab170854) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Ramos cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 146 kDa
ab170854 has not yet been referenced specifically in any publications.