Key features and details
- Rabbit polyclonal to SF3B14
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SF3B14 antibody
See all SF3B14 primary antibodies
DescriptionRabbit polyclonal to SF3B14
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthesized peptide derived from the C-terminal region of human SF3B14
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 14 kDa.|
FunctionNecessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
- Information by UniProt
- Ht006 antibody
- P14 antibody
- PM14_HUMAN antibody
All lanes : Anti-SF3B14 antibody (ab74676) at 1/500 dilution
Lane 1 : extracts from HepG2 cells
Lane 2 : extracts from HepG2 cells with immunizing peptide at 10 µg
Lysates/proteins at 20 µg per lane.
Predicted band size: 14 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
ab74676 has not yet been referenced specifically in any publications.