Overview

  • Product name
    Anti-SF3B2 antibody
  • Description
    Mouse monoclonal to SF3B2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment: ISLGMPVGPN AHKVPPPWLI AMQRYGPPPS YPNLKIPGLN SPIPESCSFG YHAGG, corresponding to amino acids 592-646 of Human SF3B2

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab56800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 98 kDa.
IHC-P Use a concentration of 6 µg/ml.
ICC/IF Use a concentration of 60 µg/ml.

Target

  • Relevance
    SF3B2 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B2 may also be involved in the assembly of the 'E' complex. It also belongs to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • Pre mRNA splicing factor SF3b 145 kDa subunit antibody
    • SAP 145 antibody
    • SAP145 antibody
    • SF3b1 antibody
    • SF3B145 antibody
    • SF3b150 antibody
    • Spliceosome associated protein 145 antibody
    • Splicing factor 3b subunit 2 145kDa antibody
    • Splicing factor 3b subunit 2 antibody
    see all

Images

  • SF3B2 antibody (ab56800) used in immunohistochemistry at 6ug/ml on formalin fixed and paraffin embedded human kidney.
  • SF3B2 antibody (ab56800) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab56800, at 60 µg/ml staining SF3B2 in HeLa Cells by immunocytochemistry. Immunofluorescence of ab56800 to SF3B2 on HeLa cell, performed with antibody concentration at 60 ug/ml.

References

This product has been referenced in:
  • Lin-Moshier Y  et al. Re-evaluation of the role of calcium homeostasis endoplasmic reticulum protein (CHERP) in cellular calcium signaling. J Biol Chem 288:355-67 (2013). WB . Read more (PubMed: 23148228) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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