Key features and details
- Rabbit polyclonal to SF3B3
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SF3B3 antibody
See all SF3B3 primary antibodies
DescriptionRabbit polyclonal to SF3B3
Tested applicationsSuitable for: IP, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Zebrafish
Synthetic peptide within Human SF3B3 aa 1167-1217. The exact sequence is proprietary. NP_036558.3
Database link: Q15393
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysates. IHC-P: Human prostate carcinoma tissue; mouse teratoma tissue.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245640 was affinity purified using an epitope specific to SF3B3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 136 kDa.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionSubunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
Sequence similaritiesBelongs to the RSE1 family.
- Information by UniProt
- KIAA0017 antibody
- Pre mRNA splicing factor SF3b 130 kDa subunit antibody
- Pre-mRNA-splicing factor SF3b 130 kDa subunit antibody
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue (left panel) and mouse teratoma tissue (right panel) stained for SF3B3 using ab245640 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
All lanes : Anti-SF3B3 antibody (ab245640) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 136 kDa
Exposure time: 1 second
SF3B3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245640 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245640 at 0.1 µg/ml.
Lane 1: ab245640 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 1 second.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245640 has not yet been referenced specifically in any publications.