Overview

  • Product name

  • Description

    Rabbit polyclonal to SF3B3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow, Zebrafish
  • Immunogen

    Synthetic peptide within Human SF3B3 aa 1167-1217. The exact sequence is proprietary. NP_036558.3
    Database link: Q15393

  • Positive control

    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysates. IHC-P: Human prostate carcinoma tissue; mouse teratoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab245640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 136 kDa.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
  • Sequence similarities

    Belongs to the RSE1 family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA0017 antibody
    • Pre mRNA splicing factor SF3b 130 kDa subunit antibody
    • Pre-mRNA-splicing factor SF3b 130 kDa subunit antibody
    • RSE1 antibody
    • SAP 130 antibody
    • SAP130 antibody
    • SF3b130 antibody
    • SF3B3 antibody
    • SF3B3_HUMAN antibody
    • Spliceosome associated protein 130 antibody
    • Spliceosome-associated protein 130 antibody
    • Splicing factor 3b subunit 3 130kD antibody
    • Splicing factor 3B subunit 3 antibody
    • STAF130 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human prostate carcinoma tissue (left panel) and mouse teratoma tissue (right panel) stained for SF3B3 using ab245640 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.

  • All lanes : Anti-SF3B3 antibody (ab245640) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 136 kDa


    Exposure time: 1 second
  • SF3B3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245640 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245640 at 0.1 µg/ml.

    Lane 1: ab245640 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 1 second.

References

ab245640 has not yet been referenced specifically in any publications.

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