Anti-SF3B4 antibody (ab157117)
Key features and details
- Rabbit polyclonal to SF3B4
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SF3B4 antibody
See all SF3B4 primary antibodies -
Description
Rabbit polyclonal to SF3B4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide corresponding to a region within amino acids 374-424 of Human SF3B4 (NP_005841.1).
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Positive control
- HeLa, 293T, NIH 3T3 and Jurkat whole cell lysate (ab7899).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/1000. Predicted molecular weight: 44 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| Notes |
|---|
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WB
1/2000 - 1/1000. Predicted molecular weight: 44 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 426042 Chicken
- Entrez Gene: 457265 Chimpanzee
- Entrez Gene: 506123 Cow
- Entrez Gene: 483177 Dog
- Entrez Gene: 101127176 Gorilla
- Entrez Gene: 10262 Human
- Entrez Gene: 107701 Mouse
- Entrez Gene: 100157814 Pig
see all -
Alternative names
- AFD1 antibody
- Hsh49 antibody
- MGC10828 antibody
see all
Images
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Western blot - Anti-SF3B4 antibody (ab157117)All lanes : Anti-SF3B4 antibody (ab157117) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 44 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-SF3B4 antibody (ab157117)Immunoprecipitation analysis of whole cell lysate from 293T cells (1 mg for IP; 20% of IP loaded), labeling SF3B4 with ab157117 at 6 µg/mg lysate (lane 2). Immunoprecipitation of SF3B4 was also tested using an alternative Rabbit anti-SF3B4 (lane 1), or control IgG (lane 3). For blotting immunoprecipitated SF3B4, ab157117 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab157117 has not yet been referenced specifically in any publications.