Product nameAnti-SF3B4 antibody
See all SF3B4 primary antibodies
DescriptionRabbit polyclonal to SF3B4
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
- HeLa, 293T, NIH 3T3 and Jurkat whole cell lysate (ab7899).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab157117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/1000. Predicted molecular weight: 44 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
- Information by UniProt
- AFD1 antibody
- Hsh49 antibody
- MGC10828 antibody
All lanes : Anti-SF3B4 antibody (ab157117) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 44 kDa
Exposure time: 10 seconds
Immunoprecipitation analysis of whole cell lysate from 293T cells (1 mg for IP; 20% of IP loaded), labeling SF3B4 with ab157117 at 6 µg/mg lysate (lane 2). Immunoprecipitation of SF3B4 was also tested using an alternative Rabbit anti-SF3B4 (lane 1), or control IgG (lane 3). For blotting immunoprecipitated SF3B4, ab157117 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
ab157117 has not yet been referenced specifically in any publications.