Overview

  • Product name

    Anti-SF3B5 antibody
  • Description

    Rabbit polyclonal to SF3B5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide within Human SF3B5 aa 36-86. The exact sequence is proprietary.
    Database link: Q9BWJ5

  • Positive control

    • IP: HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab241211 was affinity purified using an epitope specific to SF3B5 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

Use 8 - 15 µl/mg lysate.

  • Application notes
    Is unsuitable for WB.
  • Target

    Images

    • SF3B5 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241211 at 8 µl/reaction. Western blot was performed from the immunoprecipitate using ab241211 at 1/400 dilution.

      Lane 1: ab241211 IP in HEK-293T whole cell lysate.

      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

      Detection: Chemiluminescence with exposure time of 3 minutes.

      Lysate prepared using NETN lysis buffer.

    References

    ab241211 has not yet been referenced specifically in any publications.

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