Key features and details
- Rabbit polyclonal to SF3B5
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SF3B5 antibody
DescriptionRabbit polyclonal to SF3B5
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
Synthetic peptide within Human SF3B5 aa 36-86. The exact sequence is proprietary.
Database link: Q9BWJ5
- IP: HEK-293T whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241211 was affinity purified using an epitope specific to SF3B5 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
Use 8 - 15 µl/mg lysate.
Sequence similaritiesBelongs to the SF3B5 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- MGC3133 antibody
- Pre-mRNA-splicing factor SF3b 10 kDa subunit antibody
- RP1-197L1.2 antibody
SF3B5 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241211 at 8 µl/reaction. Western blot was performed from the immunoprecipitate using ab241211 at 1/400 dilution.
Lane 1: ab241211 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
Lysate prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241211 has not yet been referenced specifically in any publications.