Overview

  • Product name

    Anti-SF4 antibody - N-terminal
    See all SF4 primary antibodies
  • Description

    Rabbit polyclonal to SF4 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human SF4 aa 595-645 (N terminal). The exact sequence is proprietary. NP_757386.2
    Database link: Q8IWZ8

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab201314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 72 kDa.

Target

Images

  • All lanes : Anti-SF4 antibody - N-terminal (ab201314) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 72 kDa


    Exposure time: 3 minutes
  • All lanes : Anti-SF4 antibody - N-terminal (ab201314) at 0.4 µg/ml

    Lane 1 : Immunoprecipitation using ab201314 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) from 293T cells prepared using NETN lysis buffer
    Lane 2 : Immunoprecipitation using control IgG

    Developed using the ECL technique.

    Predicted band size: 72 kDa


    Exposure time: 30 seconds

References

ab201314 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab201314.
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