Key features and details
- Rabbit polyclonal to SFI1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SFI1 antibody
See all SFI1 primary antibodies
DescriptionRabbit polyclonal to SFI1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Baboon
Recombinant fragment corresponding to Human SFI1 aa 1003-1242.
HSARKQPRRPHFLLEPAQSQRPQKPQEHGLGMAQPAAPSLTRPFLAEAPT ALVPHSPLPGALSSAPGPKQPPTASTGPELLLLPLSSFMPCGAAAPARVS AQRATPRDKPPVPSSLASVPDPHLLLPGDFSATRAGPGLSTAGSLDLEAE LEEIQQQLLHYQTTKQNLWSCRRQASSLRRWLELNREEPGPEDQEVEQQV QKELEQVEMQIQLLAEELQAQRQPIGACVARIQALRQALC
Database link: A8K8P3-1
- WB: HepG2 whole cell lysate. IHC-P: Human bladder cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 148, 38 kDa (predicted molecular weight: 148, 144, 138, 122, 118, 142, 137 kDa).|
- Information by UniProt
- hSFI1 antibody
- Protein SFI1 homolog antibody
- Sfi1 antibody
- SFI1_HUMAN antibody
Paraffin-embedded human bladder cancer tissue stained for SFI1 with ab230231 at 1/100 dilution in immunohistochemical analysis.
Anti-SFI1 antibody (ab230231) at 1/1000 dilution + HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Goat polyclonal to rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 148, 144, 138, 122, 118, 142, 137 kDa
Observed band size: 148,38 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230231 has not yet been referenced specifically in any publications.