Key features and details
- Rabbit polyclonal to SFI1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SFI1 antibody
See all SFI1 primary antibodies
DescriptionRabbit polyclonal to SFI1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human SFI1 aa 1003-1242.
HSARKQPRRPHFLLEPAQSQRPQKPQEHGLGMAQPAAPSLTRPFLAEAPT ALVPHSPLPGALSSAPGPKQPPTASTGPELLLLPLSSFMPCGAAAPARVS AQRATPRDKPPVPSSLASVPDPHLLLPGDFSATRAGPGLSTAGSLDLEAE LEEIQQQLLHYQTTKQNLWSCRRQASSLRRWLELNREEPGPEDQEVEQQV QKELEQVEMQIQLLAEELQAQRQPIGACVARIQALRQALC
Database link: A8K8P3-1
- WB: A549 and HepG2 whole cell lysates. IHC-P: Human bladder cancer and endometrial cancer tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 117,137,138,141,144,148 kDa.|
|IHC-P||1/20 - 1/200.|
- Information by UniProt
- hSFI1 antibody
- Protein SFI1 homolog antibody
- Sfi1 antibody
- SFI1_HUMAN antibody
All lanes : Anti-SFI1 antibody (ab230242) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 117,137,138,141,144,148 kDa
Observed band size: 148,85 kDa why is the actual band size different from the predicted?
Paraffin-embedded human bladder cancer tissue stained for SFI1 using ab230242 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human endometrial cancer tissue stained for SFI1 using ab230242 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230242 has not yet been referenced specifically in any publications.