Western blot abreview for Anti-SFPQ antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - nuclear (hESC)
Gel Running Conditions
Non-reduced Denaturing (4-20%)
Loading amount
20 µg
Specification
hESC
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-5%BSA
Dilution
1/2000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer

Detection

Detection method
ECL Regular
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 100 kDa Non-specific: 60, 65, 70, 73 kDa

Mr. Miguel Fidalgo

Verified customer

Submitted Nov 13 2015

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