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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Splicing
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RecombinantRabMAb

Recombinant Anti-SFPQ antibody [EPR11846] (ab177936)

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Western blot - Anti-SFPQ antibody [EPR11846] (ab177936)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11846] (ab177936)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11846] (ab177936)
  • Immunocytochemistry/ Immunofluorescence - Anti-SFPQ antibody [EPR11846] (ab177936)
  • Anti-SFPQ antibody [EPR11846] (ab177936)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11846] to SFPQ
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-SFPQ antibody [EPR11846]
    See all SFPQ primary antibodies
  • Description

    Rabbit monoclonal [EPR11846] to SFPQ
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human SFPQ aa 250-350. The exact sequence is proprietary.
    Database link: P23246

  • Positive control

    • Jurkat, 293T, HeLa and C6 cell lysates; Human breast carcinoma tissue; Human kidney tissue. HeLa cells.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11846
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing

Associated products

  • Alternative Versions

    • Anti-SFPQ antibody [EPR11846] - BSA and Azide free (ab250011)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab177936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 76 kDa.
ICC/IF
1/100 - 1/250.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 76 kDa.
ICC/IF
1/100 - 1/250.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1; in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends; in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Transcriptional repression is probably mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO/SF-1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.
    • Involvement in disease

      Note=A chromosomal aberration involving SFPQ may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;1)(p11.2;p34) with TFE3.
    • Sequence similarities

      Contains 2 RRM (RNA recognition motif) domains.
    • Post-translational
      modifications

      The N-terminus is blocked.
      Phosphorylated on multiple serine and threonine residues during apoptosis. In vitro phosphorylated by PKC. Phosphorylation stimulates binding to DNA and D-loop formation, but inhibits binding to RNA.
      Arg-7, Arg-9, Arg-19 and Arg-25 are dimethylated, probably to asymmetric dimethylarginine.
    • Cellular localization

      Nucleus matrix. Predominantly in nuclear matrix.
    • Target information above from: UniProt accession P23246 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6421 Human
      • Entrez Gene: 252855 Rat
      • Omim: 605199 Human
      • SwissProt: P23246 Human
      • Unigene: 355934 Human
      • Unigene: 54645 Rat
      • Alternative names

        • 100 kDa DNA pairing protein antibody
        • 100 kDa DNA-pairing protein antibody
        • 100 kDa subunit antibody
        • DNA binding p52/p100 complex 100 kDa subunit antibody
        • DNA-binding p52/p100 complex antibody
        • hPOMp100 antibody
        • Polypyrimidine tract binding protein associated splicing factor antibody
        • Polypyrimidine tract-binding protein-associated-splicing factor antibody
        • POMP100 antibody
        • PPP1R140 antibody
        • proline- and glutamine-rich antibody
        • Protein phosphatase 1 regulatory subunit 140 antibody
        • PSF antibody
        • PTB associated splicing factor antibody
        • PTB-associated-splicing factor antibody
        • Sfpq antibody
        • SFPQ_HUMAN antibody
        • Splicing factor antibody
        • Splicing factor proline and glutamine rich antibody
        • Splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated) antibody
        • Splicing factor proline/glutamine rich antibody
        see all

      Images

      • Western blot - Anti-SFPQ antibody [EPR11846] (ab177936)
        Western blot - Anti-SFPQ antibody [EPR11846] (ab177936)
        All lanes : Anti-SFPQ antibody [EPR11846] (ab177936) at 1/1000 dilution

        Lane 1 : Jurkat cell lysate
        Lane 2 : 293T cell lysate
        Lane 3 : HeLa cell lysate
        Lane 4 : C6 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 76 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11846] (ab177936)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11846] (ab177936)

        Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling SFPQ with ab177936 at 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11846] (ab177936)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11846] (ab177936)

        Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SFPQ with ab177936 at 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-SFPQ antibody [EPR11846] (ab177936)
        Immunocytochemistry/ Immunofluorescence - Anti-SFPQ antibody [EPR11846] (ab177936)

        Immunofluorescent analysis of HeLa cells labeling SFPQ with ab177936 at 1/100 dilution.

      • Anti-SFPQ antibody [EPR11846] (ab177936)
        Anti-SFPQ antibody [EPR11846] (ab177936)

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab177936? Please let us know so that we can cite the reference in this datasheet.

      ab177936 has not yet been referenced specifically in any publications.

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