Overview

  • Product name

    Anti-SFPQ antibody [EPR11847]
    See all SFPQ primary antibodies
  • Description

    Rabbit monoclonal [EPR11847] to SFPQ
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SFPQ aa 650 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P23246

  • Positive control

    • WB: Neuro-2a, NIH/3T3, RAW 264.7, HEK-293T, A431, HeLa and Jurkat whole cell lysate (ab7899). IHC-P: Human colon, pancreas and testis tissues. ICC/IF: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11847
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 100 kDa (predicted molecular weight: 76 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1; in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends; in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Transcriptional repression is probably mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO/SF-1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.
    • Involvement in disease

      Note=A chromosomal aberration involving SFPQ may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;1)(p11.2;p34) with TFE3.
    • Sequence similarities

      Contains 2 RRM (RNA recognition motif) domains.
    • Post-translational
      modifications

      The N-terminus is blocked.
      Phosphorylated on multiple serine and threonine residues during apoptosis. In vitro phosphorylated by PKC. Phosphorylation stimulates binding to DNA and D-loop formation, but inhibits binding to RNA.
      Arg-7, Arg-9, Arg-19 and Arg-25 are dimethylated, probably to asymmetric dimethylarginine.
    • Cellular localization

      Nucleus matrix. Predominantly in nuclear matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • 100 kDa DNA pairing protein antibody
      • 100 kDa DNA-pairing protein antibody
      • 100 kDa subunit antibody
      • DNA binding p52/p100 complex 100 kDa subunit antibody
      • DNA-binding p52/p100 complex antibody
      • hPOMp100 antibody
      • Polypyrimidine tract binding protein associated splicing factor antibody
      • Polypyrimidine tract-binding protein-associated-splicing factor antibody
      • POMP100 antibody
      • PPP1R140 antibody
      • proline- and glutamine-rich antibody
      • Protein phosphatase 1 regulatory subunit 140 antibody
      • PSF antibody
      • PTB associated splicing factor antibody
      • PTB-associated-splicing factor antibody
      • Sfpq antibody
      • SFPQ_HUMAN antibody
      • Splicing factor antibody
      • Splicing factor proline and glutamine rich antibody
      • Splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated) antibody
      • Splicing factor proline/glutamine rich antibody
      see all

    Images

    • All lanes : Anti-SFPQ antibody [EPR11847] (ab177149) at 1/10000 dilution

      Lane 1 : Neuro-2a (Mouse neuroblastoma cell line) cell lysate
      Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
      Lane 3 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
      Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
      Lane 5 : A431 (Human epidermoid carcinoma cell line) cell lysate
      Lane 6 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
      Lane 7 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 76 kDa

    • Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling SFPQ with ab177149 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded human testis tissue labeling SFPQ with ab177149 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling SFPQ with ab177149 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling SFPQ with ab177149 at 1/100 dilution.

    References

    This product has been referenced in:

    • An H  et al. ALS-linked FUS mutations confer loss and gain of function in the nucleus by promoting excessive formation of dysfunctional paraspeckles. Acta Neuropathol Commun 7:7 (2019). Read more (PubMed: 30642400) »
    • Menoni H  et al. The transcription-coupled DNA repair-initiating protein CSB promotes XRCC1 recruitment to oxidative DNA damage. Nucleic Acids Res 46:7747-7756 (2018). Read more (PubMed: 29955842) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293)
    Permeabilization
    Yes - 0.3% Triton-X100 in blocking buffer
    Specification
    HEK293
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Nov 30 2018

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (4-12% Bis-Tris)
    Loading amount
    25 µg
    Specification
    HEK293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jun 12 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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