Recombinant
RabMAb

Recombinant Anti-SFPQ antibody [EPR11847] - BSA and Azide free (ab246358)

Overview

  • Product name

    Anti-SFPQ antibody [EPR11847] - BSA and Azide free
    See all SFPQ primary antibodies
  • Description

    Rabbit monoclonal [EPR11847] to SFPQ - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SFPQ aa 650 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P23246

  • Positive control

    • WB: Neuro-2a, NIH/3T3, RAW 264.7, HEK-293T, A431, HeLa and Jurkat whole cell lysate (ab7899). IHC-P: Human colon, pancreas and testis tissues. ICC/IF: HeLa cells.
  • General notes

    Ab246358 is the carrier-free version of ab177149. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab246358 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11847
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab246358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 76 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1; in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends; in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Transcriptional repression is probably mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO/SF-1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.
    • Involvement in disease

      Note=A chromosomal aberration involving SFPQ may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;1)(p11.2;p34) with TFE3.
    • Sequence similarities

      Contains 2 RRM (RNA recognition motif) domains.
    • Post-translational
      modifications

      The N-terminus is blocked.
      Phosphorylated on multiple serine and threonine residues during apoptosis. In vitro phosphorylated by PKC. Phosphorylation stimulates binding to DNA and D-loop formation, but inhibits binding to RNA.
      Arg-7, Arg-9, Arg-19 and Arg-25 are dimethylated, probably to asymmetric dimethylarginine.
    • Cellular localization

      Nucleus matrix. Predominantly in nuclear matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • 100 kDa DNA pairing protein antibody
      • 100 kDa DNA-pairing protein antibody
      • 100 kDa subunit antibody
      • DNA binding p52/p100 complex 100 kDa subunit antibody
      • DNA-binding p52/p100 complex antibody
      • hPOMp100 antibody
      • Polypyrimidine tract binding protein associated splicing factor antibody
      • Polypyrimidine tract-binding protein-associated-splicing factor antibody
      • POMP100 antibody
      • PPP1R140 antibody
      • proline- and glutamine-rich antibody
      • Protein phosphatase 1 regulatory subunit 140 antibody
      • PSF antibody
      • PTB associated splicing factor antibody
      • PTB-associated-splicing factor antibody
      • Sfpq antibody
      • SFPQ_HUMAN antibody
      • Splicing factor antibody
      • Splicing factor proline and glutamine rich antibody
      • Splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated) antibody
      • Splicing factor proline/glutamine rich antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling SFPQ with ab177149 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab177149).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling SFPQ with ab177149 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab177149).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded human testis tissue labeling SFPQ with ab177149 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab177149).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling SFPQ with ab177149 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab177149).

    References

    ab246358 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab246358.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up