• Product name
  • Description
    Rabbit polyclonal to SFRP1
  • Host species
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog
  • Immunogen

    A region within synthetic peptide: KNGADCPCHQ LDNLSHHFLI MGRKVKSQYL LTAIHKWDKK NKEFKNFMKK, corresponding to internal sequence amino acids 252-301 of Human SFRP1

  • Positive control
    • Fetal kidney lysate.



Our Abpromise guarantee covers the use of ab66673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3125 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1/62500.


  • Function
    Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription.
  • Tissue specificity
    Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes.
  • Sequence similarities
    Belongs to the secreted frizzled-related protein (sFRP) family.
    Contains 1 FZ (frizzled) domain.
    Contains 1 NTR domain.
  • Domain
    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localization
    Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • Frizzled related protein 1 antibody
    • FRP 1 antibody
    • FRP antibody
    • FRP-1 antibody
    • FRP1 antibody
    • FrzA antibody
    • SARP 2 antibody
    • SARP-2 antibody
    • SARP2 antibody
    • Secreted apoptosis related protein 2 antibody
    • Secreted apoptosis-related protein 2 antibody
    • Secreted frizzled related protein 1 antibody
    • Secreted frizzled related protein antibody
    • Secreted frizzled-related protein 1 antibody
    • SFRP 1 antibody
    • sFRP-1 antibody
    • SFRP1 antibody
    • SFRP1_HUMAN antibody
    see all


  • Anti-SFRP1 antibody (ab66673) at 0.3125 µg/ml (in 5% skim milk / PBS buffer) + Fetal kidney lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 35 kDa
    Observed band size: 35 kDa
    Additional bands at: 39 kDa. We are unsure as to the identity of these extra bands.

    Gel concentration 12%


This product has been referenced in:
  • Qiao B  et al. MicroRNA-27a-3p Modulates the Wnt/ß-Catenin Signaling Pathway to Promote Epithelial-Mesenchymal Transition in Oral Squamous Carcinoma Stem Cells by Targeting SFRP1. Sci Rep 7:44688 (2017). WB ; Human . Read more (PubMed: 28425477) »

See 1 Publication for this product

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