Overview

  • Product name

  • Description

    Rabbit polyclonal to SFRP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human SFRP1 aa 250 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab101296)

  • Positive control

    • This antibody gave a positive signal in Human heart tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 35 kDa).

Target

  • Function

    Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription.
  • Tissue specificity

    Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes.
  • Sequence similarities

    Belongs to the secreted frizzled-related protein (sFRP) family.
    Contains 1 FZ (frizzled) domain.
    Contains 1 NTR domain.
  • Domain

    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localization

    Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding.
  • Information by UniProt
  • Database links

  • Alternative names

    • Frizzled related protein 1 antibody
    • FRP 1 antibody
    • FRP antibody
    • FRP-1 antibody
    • FRP1 antibody
    • FrzA antibody
    • SARP 2 antibody
    • SARP-2 antibody
    • SARP2 antibody
    • Secreted apoptosis related protein 2 antibody
    • Secreted apoptosis-related protein 2 antibody
    • Secreted frizzled related protein 1 antibody
    • Secreted frizzled related protein antibody
    • Secreted frizzled-related protein 1 antibody
    • SFRP 1 antibody
    • sFRP-1 antibody
    • SFRP1 antibody
    • SFRP1_HUMAN antibody
    see all

Images

  • Anti-SFRP1 antibody (ab84003) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 35 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 52 kDa, 68 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes


    The band observed at 32 kDa could potentially be a cleaved form of SFRP1 due to the presence of a 31 amino acid signal peptide.

References

ab84003 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

I understand that you may be concerned about non-specific binding of the antibody, therefore please find the immunogen sequence below: ********************** With this immunogen information you will be able to do your on BLAST searches in case you do see non-specific binding. Please let me know if there is anything else I can help you with.

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Answer

Thank you for your reply. Based on the sequences you sent, there is a 100% homology between the ground squirrel sequence and the immunogen used for ab84003. Please find the testing discount details below. As this antibody has only been tested on human samples, they would be the best positive control, however based on sequence homology, mouse samples should work well with this antibody. Please let me know if there is anything else I can help you with. DISCOUNT CODE: *********** Expiration date: ************ I am very pleased to hear you would like to accept our offer and test ab84003 in ground squirrel. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for squirrel and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for your reply. Unfortunately the FASTA file formats you sent would not open on my computer, but since you said that there was a 93% level of homology between the squirrel and the human sequence, then I can offer you our testing discount program. Please see the details below: As ab84003 has not been tested in ground squirrel, I can offer a discount off a future purchase if you buy ab84003 now, test it in ground squirrel and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab84003 in ground squirrel. I will then send a discount code. This code must be issued before purchasing ab84003 so please wait for my reply before ordering. 2. Purchase ab84003 either by phone, fax, or online (www.abcam.com). 3. Test it in ground squirrel. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODYordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab84003 turns out to be unsuitable for ground squirrel, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting Abcam. Under our Abpromise, we guarantee our antibodies to work as stated on the datasheet, and if it does not works as stated then we would be happy to replace or refund the antibody. In your case, as this antibody has not been tested against the thirteen-lined ground squirrel, we cannot guarantee that it will work for you. I have tried looking for the SFRP1 sequence for the ground squirrel, but I have not been able to find it. If you have a link to that sequence, then I could do an alignment between that sequence and the immunogen sequence for ab84003 and see if there is a good chance of success. If there is a chance of the antibody working against squirrel tissue, then I could issue you a testing discount code. I look forward to you response.

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Question
Answer

DISCOUNT CODE: [XXXXX] Expiration date: [07/03/2012] I am very pleased to hear you would like to accept our offer and test ab[87541] in [ICC/IF]. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer, please submit an Abreview for [ICC/IF] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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