Key features and details
- Rabbit polyclonal to SFRP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SFRP1 antibody
See all SFRP1 primary antibodies
DescriptionRabbit polyclonal to SFRP1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Synthetic peptide corresponding to Human SFRP1 aa 250 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human heart tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab84003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 35 kDa).|
FunctionSoluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription.
Tissue specificityWidely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes.
Sequence similaritiesBelongs to the secreted frizzled-related protein (sFRP) family.
Contains 1 FZ (frizzled) domain.
Contains 1 NTR domain.
DomainThe FZ domain is involved in binding with Wnt ligands.
Cellular localizationSecreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding.
- Information by UniProt
- Frizzled related protein 1 antibody
- FRP 1 antibody
- FRP antibody
Anti-SFRP1 antibody (ab84003) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Additional bands at: 52 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The band observed at 32 kDa could potentially be a cleaved form of SFRP1 due to the presence of a 31 amino acid signal peptide.
ab84003 has not yet been referenced specifically in any publications.