• Product name
  • Description
    Rabbit polyclonal to SFRP1
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human SFRP1.

    (Peptide available as ab101296.)

  • Positive control
    • This antibody gave a positive signal in Human heart tissue lysate.



Our Abpromise guarantee covers the use of ab84003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 35 kDa).


  • Function
    Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription.
  • Tissue specificity
    Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes.
  • Sequence similarities
    Belongs to the secreted frizzled-related protein (sFRP) family.
    Contains 1 FZ (frizzled) domain.
    Contains 1 NTR domain.
  • Domain
    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localization
    Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • Frizzled related protein 1 antibody
    • FRP 1 antibody
    • FRP antibody
    • FRP-1 antibody
    • FRP1 antibody
    • FrzA antibody
    • SARP 2 antibody
    • SARP-2 antibody
    • SARP2 antibody
    • Secreted apoptosis related protein 2 antibody
    • Secreted apoptosis-related protein 2 antibody
    • Secreted frizzled related protein 1 antibody
    • Secreted frizzled related protein antibody
    • Secreted frizzled-related protein 1 antibody
    • SFRP 1 antibody
    • sFRP-1 antibody
    • SFRP1 antibody
    • SFRP1_HUMAN antibody
    see all


  • Anti-SFRP1 antibody (ab84003) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 35 kDa
    Observed band size: 32 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 52 kDa, 68 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 12 minutes

    The band observed at 32 kDa could potentially be a cleaved form of SFRP1 due to the presence of a 31 amino acid signal peptide.


ab84003 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

I understand that you may be concerned about non-specific binding of the antibody, therefore please find the immunogen sequence below: ********************** With this immunogen information you will be able to do your on BLAST searches in case you do s...

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Thank you for your reply. Based on the sequences you sent, there is a 100% homology between the ground squirrel sequence and the immunogen used for ab84003. Please find the testing discount details below. As this antibody has only been tested on human...

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Thank you for your reply. Unfortunately the FASTA file formats you sent would not open on my computer, but since you said that there was a 93% level of homology between the squirrel and the human sequence, then I can offer you our testing discount prog...

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Thank you for contacting Abcam. Under our Abpromise, we guarantee our antibodies to work as stated on the datasheet, and if it does not works as stated then we would be happy to replace or refund the antibody. In your case, as this antibody has not bee...

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DISCOUNT CODE: [XXXXX] Expiration date: [07/03/2012] I am very pleased to hear you would like to accept our offer and test ab[87541] in [ICC/IF]. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this off...

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