Recombinant
RabMAb

Recombinant Anti-SFRP1 antibody [EPR7003] (ab126613)

Reviews (1) Submit a question References (12)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7003] to SFRP1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-SFRP1 antibody [EPR7003]
    See all SFRP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7003] to SFRP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide, corresponding to residues in Human SFRP1. (Uniprot: Q8N474).

  • Positive control

    • Human heart, MDA-MB-435 and A375 lysates; Human heart muscle tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab126613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.

    • Target

    • Function

      Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription.

    • Tissue specificity

      Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes.

    • Sequence similarities

      Belongs to the secreted frizzled-related protein (sFRP) family.
      Contains 1 FZ (frizzled) domain.
      Contains 1 NTR domain.

    • Domain

      The FZ domain is involved in binding with Wnt ligands.

    • Cellular localization

      Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding.
    • Information by UniProt

    • Database links

      • Alternative names

        • Frizzled related protein 1 antibody
        • FRP 1 antibody
        • FRP antibody
        • FRP-1 antibody
        • FRP1 antibody
        • FrzA antibody
        • SARP 2 antibody
        • SARP-2 antibody
        • SARP2 antibody
        • Secreted apoptosis related protein 2 antibody
        • Secreted apoptosis-related protein 2 antibody
        • Secreted frizzled related protein 1 antibody
        • Secreted frizzled related protein antibody
        • Secreted frizzled-related protein 1 antibody
        • SFRP 1 antibody
        • sFRP-1 antibody
        • SFRP1 antibody
        • SFRP1_HUMAN antibody
        see all

      Images

      • Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613)
        Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613)
        Anti-SFRP1 antibody [EPR7003] (ab126613) at 1/1000 dilution (purified) + MDA-MB-435S (Human mammary gland ductal carcinoma melanocyte) whole cell lysates at 15 µg

        Secondary
        Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

        Predicted band size: 35 kDa



        Blocking and diluting buffer: 5% NFDM/TBST

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP1 antibody [EPR7003] (ab126613)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP1 antibody [EPR7003] (ab126613)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling SFRP1 with Purified ab126613 at 1:50 dilution (8 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

      • Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613)
        Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613)
        All lanes : Anti-SFRP1 antibody [EPR7003] (ab126613) at 1/5000 dilution (purified)

        Lane 1 : Human fetal kidney lysates
        Lane 2 : Human fetal heart lysates

        Lysates/proteins at 15 µg per lane.

        Secondary
        All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

        Predicted band size: 35 kDa
        Observed band size: 36 kDa
        why is the actual band size different from the predicted?



        Blocking and diluting buffer: 5% NFDM/TBST

      • Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613)
        Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613)
        All lanes : Anti-SFRP1 antibody [EPR7003] (ab126613) at 1/1000 dilution (unpurified)

        Lane 1 : Human heart lysate
        Lane 2 : MDA-MB-435 lysate
        Lane 3 : A375 lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 35 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP1 antibody [EPR7003] (ab126613)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP1 antibody [EPR7003] (ab126613)

        Unpurified ab126613, at 1/50 dilution, staining SFRP1 in Formalin-fixed, Paraffin-embedded Human heart muscle tissue by Immunohistochemistry.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Other - Anti-SFRP1 antibody [EPR7003] (ab126613)
        Other - Anti-SFRP1 antibody [EPR7003] (ab126613)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      Publishing research using ab126613? Please let us know so that we can cite the reference in this datasheet.

      ab126613 has been referenced in 12 publications.

      • Peng JX  et al. sFRP1 exerts effects on gastric cancer cells through GSK3ß/Rac1-mediated restraint of TGFß/Smad3 signaling. Oncol Rep 41:224-234 (2019). PubMed: 30542739
      • Esteve P  et al. Elevated levels of Secreted-Frizzled-Related-Protein 1 contribute to Alzheimer's disease pathogenesis. Nat Neurosci 22:1258-1268 (2019). PubMed: 31308530
      • Song XL  et al. miR-1301-3p promotes prostate cancer stem cell expansion by targeting SFRP1 and GSK3ß. Biomed Pharmacother 99:369-374 (2018). WB, RT-PCR . PubMed: 29358129
      • Tripathy A  et al. The molecular connection of histopathological heterogeneity in hepatocellular carcinoma: A role of Wnt and Hedgehog signaling pathways. PLoS One 13:e0208194 (2018). PubMed: 30513115
      • Takata N  et al. Self-patterning of rostral-caudal neuroectoderm requires dual role of Fgf signaling for localized Wnt antagonism. Nat Commun 8:1339 (2017). PubMed: 29109536
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Western blot abreview for Anti-SFRP1 antibody [EPR7003]

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (Mouse lung fibroblasts (MLg2908))
      Gel Running Conditions
      Reduced Denaturing (10%)
      Loading amount
      30 µg
      Specification
      Mouse lung fibroblasts (MLg2908)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
      Read More

      Arunima Seal

      Verified customer

      Submitted Sep 05 2016

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com