Recombinant Anti-SFRP1 antibody [EPR7003] (ab126613)
- Datasheet
- References (11)
- Protocols
Overview
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Product name
Anti-SFRP1 antibody [EPR7003]
See all SFRP1 primary antibodies -
Description
Rabbit monoclonal [EPR7003] to SFRP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to residues in Human SFRP1. (Uniprot: Q8N474).
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Positive control
- Human heart, MDA-MB-435 and A375 lysates; Human heart muscle tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.01 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7003 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab126613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 35 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription. -
Tissue specificity
Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes. -
Sequence similarities
Belongs to the secreted frizzled-related protein (sFRP) family.
Contains 1 FZ (frizzled) domain.
Contains 1 NTR domain. -
Domain
The FZ domain is involved in binding with Wnt ligands. -
Cellular localization
Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding. - Information by UniProt
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Database links
- Entrez Gene: 6422 Human
- Omim: 604156 Human
- SwissProt: Q8N474 Human
- Unigene: 213424 Human
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Alternative names
- Frizzled related protein 1 antibody
- FRP 1 antibody
- FRP antibody
see all
Images
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![Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613)](https://www.abcam.com/ps/products/126/ab126613/Images/ab126613-296774-anti-sfrp1-antibody-epr7003-western-blot.jpg)
Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613)Anti-SFRP1 antibody [EPR7003] (ab126613) at 1/1000 dilution (purified) + MDA-MB-435S (Human mammary gland ductal carcinoma melanocyte) whole cell lysates at 15 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 35 kDaBlocking and diluting buffer: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP1 antibody [EPR7003] (ab126613)](https://www.abcam.com/ps/products/126/ab126613/Images/ab126613-297707-anti-sfrp1-antibody-epr7003-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP1 antibody [EPR7003] (ab126613)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling SFRP1 with Purified ab126613 at 1:50 dilution (8 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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![Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613)](https://www.abcam.com/ps/products/126/ab126613/Images/ab126613-296772-anti-sfrp1-antibody-epr7003-western-blot.jpg)
Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613)All lanes : Anti-SFRP1 antibody [EPR7003] (ab126613) at 1/5000 dilution (purified)
Lane 1 : Human fetal kidney lysates
Lane 2 : Human fetal heart lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 35 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613)](https://www.abcam.com/ps/products/126/ab126613/Images/ab126613-140596-anti-sfrp1-antibody-epr7003-western-blot.jpg)
Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613)All lanes : Anti-SFRP1 antibody [EPR7003] (ab126613) at 1/1000 dilution (unpurified)
Lane 1 : Human heart lysate
Lane 2 : MDA-MB-435 lysate
Lane 3 : A375 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 35 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP1 antibody [EPR7003] (ab126613)](https://www.abcam.com/ps/products/126/ab126613/Images/ab126613-140595-anti-sfrp1-antibody-epr7003-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP1 antibody [EPR7003] (ab126613)Unpurified ab126613, at 1/50 dilution, staining SFRP1 in Formalin-fixed, Paraffin-embedded Human heart muscle tissue by Immunohistochemistry.
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![Other - Anti-SFRP1 antibody [EPR7003] (ab126613)](https://www.abcam.com/ps/products/126/ab126613/Images/ab126613-140594-anti-sfrp1-antibody-epr7003.jpg)
Other - Anti-SFRP1 antibody [EPR7003] (ab126613)
Protocols
Datasheets and documents
References
This product has been referenced in:
- Peng JX et al. sFRP1 exerts effects on gastric cancer cells through GSK3ß/Rac1-mediated restraint of TGFß/Smad3 signaling. Oncol Rep 41:224-234 (2019). Read more (PubMed: 30542739) »
- Song XL et al. miR-1301-3p promotes prostate cancer stem cell expansion by targeting SFRP1 and GSK3ß. Biomed Pharmacother 99:369-374 (2018). WB, RT-PCR . Read more (PubMed: 29358129) »
