Recombinant
RabMAb

Recombinant Anti-SFRP1 antibody [EPR7003] - BSA and Azide free (ab240023)

Overview

  • Product name

    Anti-SFRP1 antibody [EPR7003] - BSA and Azide free
    See all SFRP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7003] to SFRP1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SFRP1 aa 1-100. The exact sequence is proprietary.

  • General notes

    ab240023 is the carrier-free version of ab126613 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab240023 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription.
    • Tissue specificity

      Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes.
    • Sequence similarities

      Belongs to the secreted frizzled-related protein (sFRP) family.
      Contains 1 FZ (frizzled) domain.
      Contains 1 NTR domain.
    • Domain

      The FZ domain is involved in binding with Wnt ligands.
    • Cellular localization

      Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding.
    • Information by UniProt
    • Database links

    • Alternative names

      • Frizzled related protein 1 antibody
      • FRP 1 antibody
      • FRP antibody
      • FRP-1 antibody
      • FRP1 antibody
      • FrzA antibody
      • SARP 2 antibody
      • SARP-2 antibody
      • SARP2 antibody
      • Secreted apoptosis related protein 2 antibody
      • Secreted apoptosis-related protein 2 antibody
      • Secreted frizzled related protein 1 antibody
      • Secreted frizzled related protein antibody
      • Secreted frizzled-related protein 1 antibody
      • SFRP 1 antibody
      • sFRP-1 antibody
      • SFRP1 antibody
      • SFRP1_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling SFRP1 with Purified ab126613 at 1:50 dilution (8 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126613).

    • Unpurified ab126613, at 1/50 dilution, staining SFRP1 in Formalin-fixed, Paraffin-embedded Human heart muscle tissue by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126613).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126613).

    References

    ab240023 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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