Product nameAnti-SFRP2 antibody - C-terminal
See all SFRP2 primary antibodies
DescriptionRabbit polyclonal to SFRP2 - C-terminal
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog, Zebrafish
Synthetic peptide, corresponding to a region within C terminal amino acids 231-295 of Human SFRP2.
- 293T, A431, H1299, HeLa, HepG2, Molt 4 and Raji cell lysates; A431 cells; MCF7 xenograft
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% CMIT/MIT 3:1
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).|
|WB||1/500 - 1/3000. Predicted molecular weight: 33 kDa.|
FunctionSoluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.
Tissue specificityExpressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon.
Sequence similaritiesBelongs to the secreted frizzled-related protein (sFRP) family.
Contains 1 FZ (frizzled) domain.
Contains 1 NTR domain.
DomainThe FZ domain is involved in binding with Wnt ligands.
- Information by UniProt
- AI851596 antibody
- Frizzled related protein 2 antibody
- FRP 2 antibody
All lanes : Anti-SFRP2 antibody - C-terminal (ab137560) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : Hela whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 33 kDa
12% SDS PAGE
Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labelling SFRP2 with ab137560 at 1/200 dilution. Lower image is merged with DNA probe.
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft labelling SFRP2 with ab137560 at 1/500 dilution.
This product has been referenced in:
- Shi J et al. Overexpression of LINC00261 inhibits non-small cell lung cancer cells progression by interacting with miR-522-3p and suppressing Wnt signaling. J Cell Biochem 120:18378-18387 (2019). Read more (PubMed: 31190356) »
- Cai J et al. Simultaneous overactivation of Wnt/ß-catenin and TGFß signalling by miR-128-3p confers chemoresistance-associated metastasis in NSCLC. Nat Commun 8:15870 (2017). Read more (PubMed: 28627514) »