Overview

  • Product name

    Anti-SFRP2 antibody - C-terminal
    See all SFRP2 primary antibodies
  • Description

    Rabbit polyclonal to SFRP2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 231-295 of Human SFRP2.

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, Molt 4 and Raji cell lysates; A431 cells; MCF7 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab137560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
WB 1/500 - 1/3000. Predicted molecular weight: 33 kDa.

Target

  • Function

    Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.
  • Tissue specificity

    Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon.
  • Sequence similarities

    Belongs to the secreted frizzled-related protein (sFRP) family.
    Contains 1 FZ (frizzled) domain.
    Contains 1 NTR domain.
  • Domain

    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI851596 antibody
    • Frizzled related protein 2 antibody
    • FRP 2 antibody
    • FRP-2 antibody
    • FRP2 antibody
    • MGC128911 antibody
    • MGC153618 antibody
    • MGC53344 antibody
    • SARP 1 antibody
    • SARP-1 antibody
    • SARP1 antibody
    • SDF 5 antibody
    • SDF5 antibody
    • Secreted apoptosis related protein 1 antibody
    • Secreted apoptosis-related protein 1 antibody
    • Secreted frizzled related protein 2 antibody
    • Secreted frizzled-related protein 2 antibody
    • sFRP 2 antibody
    • sFRP-2 antibody
    • SFRP2 antibody
    • Sfrp2 protein antibody
    • SFRP2_HUMAN antibody
    see all

Images

  • All lanes : Anti-SFRP2 antibody - C-terminal (ab137560) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : Hela whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 33 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labelling SFRP2 with ab137560 at 1/200 dilution. Lower image is merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded MCF7 xenograft labelling SFRP2 with ab137560 at 1/500 dilution.

References

This product has been referenced in:

  • Cai J  et al. Simultaneous overactivation of Wnt/ß-catenin and TGFß signalling by miR-128-3p confers chemoresistance-associated metastasis in NSCLC. Nat Commun 8:15870 (2017). Read more (PubMed: 28627514) »
  • Kaur A  et al. sFRP2 in the aged microenvironment drives melanoma metastasis and therapy resistance. Nature 532:250-4 (2016). ELISA . Read more (PubMed: 27042933) »
See all 2 Publications for this product

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