Overview

  • Product name

  • Description

    Goat polyclonal to SFRP4
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to internal sequence amino acids of Human SFRP4.

  • Positive control

    • Human Skeletal Muscle (Interstitial Cells) tissue; Human uterus lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P Use a concentration of 3.75 µg/ml.

Target

  • Function

    Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.
  • Tissue specificity

    Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Shows moderate to strong expression in ovarian tumors with expression increasing as the tumor stage increases. In ovarian tumors, expression levels are inversely correlated with expression of CTNNB1 (at protein level).
  • Sequence similarities

    Belongs to the secreted frizzled-related protein (sFRP) family.
    Contains 1 FZ (frizzled) domain.
    Contains 1 NTR domain.
  • Domain

    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localization

    Secreted. Cytoplasmic in ovarian tumor cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • Frizzled protein antibody
    • Frizzled protein human endometrium antibody
    • FRP 4 antibody
    • FRP4 antibody
    • FrpHE antibody
    • human endometrium antibody
    • PYL antibody
    • Secreted frizzled-related protein 4 antibody
    • sFRP-4 antibody
    • Sfrp4 antibody
    • SFRP4_HUMAN antibody
    see all

Images

  • ab122905, at 3.75 µg/ml, staining SFRP4 in Formalin-fixed, Paraffin-embedded Human Skeletal Muscle (interstitial cells) tissue by Immunohistochemistry.
  • Anti-SFRP4 antibody (ab122905) at 0.3 µg/ml + Human uterus lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 40 kDa

References

This product has been referenced in:

  • Zhang Z  et al. Association of Wnt2 and sFRP4 expression in the third trimester placenta in women with severe preeclampsia. Reprod Sci 20:981-9 (2013). Read more (PubMed: 23322712) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab122905.
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