Key features and details
- Goat polyclonal to SFRP4
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SFRP4 antibody
See all SFRP4 primary antibodies
DescriptionGoat polyclonal to SFRP4
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
Synthetic peptide conjugated to KLH, corresponding to internal sequence amino acids of Human SFRP4.
- Human Skeletal Muscle (Interstitial Cells) tissue; Human uterus lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab122905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).|
|IHC-P||Use a concentration of 3.75 µg/ml.|
FunctionSoluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.
Tissue specificityExpressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Shows moderate to strong expression in ovarian tumors with expression increasing as the tumor stage increases. In ovarian tumors, expression levels are inversely correlated with expression of CTNNB1 (at protein level).
Sequence similaritiesBelongs to the secreted frizzled-related protein (sFRP) family.
Contains 1 FZ (frizzled) domain.
Contains 1 NTR domain.
DomainThe FZ domain is involved in binding with Wnt ligands.
Cellular localizationSecreted. Cytoplasmic in ovarian tumor cells.
- Information by UniProt
- Frizzled protein antibody
- Frizzled protein human endometrium antibody
- FRP 4 antibody
ab122905, at 3.75 µg/ml, staining SFRP4 in Formalin-fixed, Paraffin-embedded Human Skeletal Muscle (interstitial cells) tissue by Immunohistochemistry.
Anti-SFRP4 antibody (ab122905) at 0.3 µg/ml + Human uterus lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 40 kDa
ab122905 has been referenced in 2 publications.
- Fitzgerald HC et al. Idiopathic infertility in women is associated with distinct changes in proliferative phase uterine fluid proteins. Biol Reprod 98:752-764 (2018). PubMed: 29546322
- Zhang Z et al. Association of Wnt2 and sFRP4 expression in the third trimester placenta in women with severe preeclampsia. Reprod Sci 20:981-9 (2013). PubMed: 23322712