Recombinant Anti-SFRP4 antibody [EPR9389] (ab154167)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9389] to SFRP4
- Suitable for: WB, IHC-P, Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-SFRP4 antibody [EPR9389]
See all SFRP4 primary antibodies -
Description
Rabbit monoclonal [EPR9389] to SFRP4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra), ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human ovary cancer, uterus cancer, MCF7 and 293T lysates; Human colon tissue, Human ovarian carcinoma tissue. ICC/IF: A549 cells, RAW264.7 cells. WB: Human ovary cancer tissue lysate, MCF7, THP-1 cell lysate, Mouse ovary tissue lysate, bEnd.3, HC11, Raw 264.7 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9389 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
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IHC-P | (1) |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. -
Tissue specificity
Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Shows moderate to strong expression in ovarian tumors with expression increasing as the tumor stage increases. In ovarian tumors, expression levels are inversely correlated with expression of CTNNB1 (at protein level). -
Sequence similarities
Belongs to the secreted frizzled-related protein (sFRP) family.
Contains 1 FZ (frizzled) domain.
Contains 1 NTR domain. -
Domain
The FZ domain is involved in binding with Wnt ligands. -
Cellular localization
Secreted. Cytoplasmic in ovarian tumor cells. - Information by UniProt
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Database links
- Entrez Gene: 6424 Human
- Entrez Gene: 20379 Mouse
- Entrez Gene: 89803 Rat
- Omim: 606570 Human
- SwissProt: Q6FHJ7 Human
- SwissProt: Q9Z1N6 Mouse
- SwissProt: Q9JLS4 Rat
- Unigene: 658169 Human
see all -
Alternative names
- Frizzled protein antibody
- Frizzled protein human endometrium antibody
- FRP 4 antibody
see all
Images
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling SFRP4 with ab154167 at 1/200 (6.805 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed seondary antibody used at 1/1000 diluting (2 µg/ml)(Green). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The Nuclear counterstain was (Blue).
Confocal image showing positive staining in RAW 264.7 cell line.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). -
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labelling SFRP4 with ab154167 at 1/500 (2.722 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed seondary antibody used at 1/1000 diluting (2 µg/ml)(Green). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The Nuclear counterstain was (Blue).
Confocal image showing positive staining in A549 cell line.
Negative control: T-47D.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). -
All lanes : Anti-SFRP4 antibody [EPR9389] (ab154167) at 1/1000 dilution
Lane 1 : Raw 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cell culture supernatant at 10 µl
Lane 2 : THP-1 (human monocytic leukemia monocyte) cell culture supernatant at 10 µl
Lane 3 : Blank control
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-SFRP4 antibody [EPR9389] (ab154167) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma epithelial cell) while cell lysate
Lane 2 : T-47D (human ductal breast epithelial tumor epithelial cell) while cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: T-47D.
In Western blot, anti-Vinculin antibody (ab129002) staining at 1/10, 000 dilution. -
All lanes : Anti-SFRP4 antibody [EPR9389] (ab154167) at 1/1000 dilution
Lane 1 : Human ovary cancer tissue lysate
Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell) while cell lysate
Lane 3 : THP-1 (human monocytic leukemia monocyte) while cell lysate
Lane 4 : Mouse ovary tissue lysate
Lane 5 : bEnd.3 (mouse brain endothelial cell) while cell lysate
Lane 6 : HC11 (mouse mammary gland epithelial cell) while cell lysate
Lane 7 : Raw 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) while cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, anti-Vinculin antibody (ab129002) staining at 1/10, 000 dilution.
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All lanes : Anti-SFRP4 antibody [EPR9389] (ab154167) at 1/1000 dilution
Lane 1 : Human ovary cancer lysate
Lane 2 : Human uterus cancer lysate
Lane 3 : MCF7 lysate
Lane 4 : 293T lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 40 kDa -
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SFRP4 with ab154167 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling SFRP4 with unpurified ab154167 at 1/130 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SFRP4 with ab154167 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab154167 has been referenced in 15 publications.
- Adekiya TA et al. In Vivo Evaluation of an Antibody-Functionalized Lipoidal Nanosystem for Schistosomiasis Intervention. Pharmaceutics 14:N/A (2022). PubMed: 35893786
- Jiang H et al. lncRNAS56464.1 as a ceRNA promotes the proliferation of fibroblast-like synoviocytes in experimental arthritis via the Wnt signaling pathway and sponges miR-152-3p. Int J Mol Med 47:N/A (2021). PubMed: 33448322
- Leow MKS et al. Paraneoplastic Secretion of Multiple Phosphatonins From a Deep Fibrous Histiocytoma Causing Oncogenic Osteomalacia. J Clin Endocrinol Metab 106:e2299-e2308 (2021). PubMed: 33462615
- Li W et al. MicroRNA-137-mediated lysine demethylase 4A regulates the recovery of spinal cord injury via the SFRP4-Wnt/ß-Catenin axis. Int J Neurosci N/A:1-14 (2021). PubMed: 33499717
- Li N et al. MeCP2 attenuates cardiomyocyte hypoxia/reperfusion-induced injury via regulation of the SFRP4/Wnt/ß-catenin axis. Biomarkers 26:363-370 (2021). PubMed: 33726573