Recombinant
RabMAb

Recombinant Anti-SFRP4 antibody [EPR9389] (ab154167)

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Overview

  • Product name

    Anti-SFRP4 antibody [EPR9389]
    See all SFRP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR9389] to SFRP4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide, corresponding to residues within Human SFRP4 (UniProt: Q6FHJ7).

  • Positive control

    • Human ovary cancer, uterus cancer, MCF7 and 293T lysates; Human colon tissue, Human ovarian carcinoma tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR9389

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.

    • Tissue specificity

      Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Shows moderate to strong expression in ovarian tumors with expression increasing as the tumor stage increases. In ovarian tumors, expression levels are inversely correlated with expression of CTNNB1 (at protein level).

    • Sequence similarities

      Belongs to the secreted frizzled-related protein (sFRP) family.
      Contains 1 FZ (frizzled) domain.
      Contains 1 NTR domain.

    • Domain

      The FZ domain is involved in binding with Wnt ligands.

    • Cellular localization

      Secreted. Cytoplasmic in ovarian tumor cells.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Frizzled protein antibody
      • Frizzled protein human endometrium antibody
      • FRP 4 antibody
      • FRP4 antibody
      • FrpHE antibody
      • human endometrium antibody
      • PYL antibody
      • Secreted frizzled-related protein 4 antibody
      • sFRP-4 antibody
      • Sfrp4 antibody
      • SFRP4_HUMAN antibody
      see all

    Images

    • Western blot - Anti-SFRP4 antibody [EPR9389] (ab154167)
      Western blot - Anti-SFRP4 antibody [EPR9389] (ab154167)
      All lanes : Anti-SFRP4 antibody [EPR9389] (ab154167) at 1/1000 dilution

      Lane 1 : Human ovary cancer lysate
      Lane 2 : Human uterus cancer lysate
      Lane 3 : MCF7 lysate
      Lane 4 : 293T lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 40 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP4 antibody [EPR9389] (ab154167)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP4 antibody [EPR9389] (ab154167)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SFRP4 with ab154167 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-SFRP4 antibody [EPR9389] (ab154167)
      Flow Cytometry - Anti-SFRP4 antibody [EPR9389] (ab154167)

      Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling SFRP4 with unpurified ab154167 at 1/130 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP4 antibody [EPR9389] (ab154167)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP4 antibody [EPR9389] (ab154167)

      Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SFRP4 with ab154167 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Zhou J  et al. Dynamic decreased expression and hypermethylation of secreted frizzled-related protein 1 and 4 over the course of pulmonary fibrosis in mice. Life Sci 218:241-252 (2019). Read more (PubMed: 30586565) »
    • Bläuer M  et al. Wnt/ß-catenin signalling plays diverse functions during the process of fibrotic remodelling in the exocrine pancreas. Pancreatology 19:252-257 (2019). Read more (PubMed: 30792046) »
    See all 7 Publications for this product

    Publishing research using ab154167? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SFRP4 antibody [EPR9389]

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step
    Other
    Sample
    Human Tissue sections (tooth)
    Specification
    tooth
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 13 2014

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