Recombinant
RabMAb

Recombinant Anti-SFRP4 antibody [EPR9389] - BSA and Azide free (ab240126)

Overview

  • Product name

    Anti-SFRP4 antibody [EPR9389] - BSA and Azide free
    See all SFRP4 primary antibodies
  • Description

    Rabbit monoclonal [EPR9389] to SFRP4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SFRP4 aa 250-350. The exact sequence is proprietary.

  • General notes

    Ab240126 is the carrier-free version of ab154167. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240126 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9389
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab240126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.
    • Tissue specificity

      Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Shows moderate to strong expression in ovarian tumors with expression increasing as the tumor stage increases. In ovarian tumors, expression levels are inversely correlated with expression of CTNNB1 (at protein level).
    • Sequence similarities

      Belongs to the secreted frizzled-related protein (sFRP) family.
      Contains 1 FZ (frizzled) domain.
      Contains 1 NTR domain.
    • Domain

      The FZ domain is involved in binding with Wnt ligands.
    • Cellular localization

      Secreted. Cytoplasmic in ovarian tumor cells.
    • Information by UniProt
    • Database links

    • Alternative names

      • Frizzled protein antibody
      • Frizzled protein human endometrium antibody
      • FRP 4 antibody
      • FRP4 antibody
      • FrpHE antibody
      • human endometrium antibody
      • PYL antibody
      • Secreted frizzled-related protein 4 antibody
      • sFRP-4 antibody
      • Sfrp4 antibody
      • SFRP4_HUMAN antibody
      see all

    Images

    • Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling SFRP4 with unpurified ab154167 at 1/130 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154167).

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SFRP4 with ab154167 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154167).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SFRP4 with ab154167 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154167).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab240126 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab240126.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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