Overview

  • Product name

    Anti-SFRS14/SUGP2 antibody
    See all SFRS14/SUGP2 primary antibodies
  • Description

    Rabbit polyclonal to SFRS14/SUGP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human SFRS14/SUGP2 (internal sequence). The exact sequence is proprietary.
    Database link: Q8IX01

  • Positive control

    • WB: HeLa nuclear extract; HeLa, Neuro-2a and C2C12 whole cell extracts. IP: HeLa whole cell extract.
  • General notes

     This product was previously labelled as SFRS14

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 120 kDa.

Target

  • Function

    May play a role in mRNA splicing.
  • Tissue specificity

    Detected in adult testis, and in fetal brain and kidney.
  • Sequence similarities

    Contains 1 G-patch domain.
    Contains 2 SURP motif repeats.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Arginine/serine rich splicing factor 14 antibody
    • arginine/serine-rich 14 antibody
    • Arginine/serine-rich-splicing factor 14 antibody
    • DKFZp779L2418 antibody
    • KIAA0365 antibody
    • Splicing factor antibody
    • Splicing factor, arginine/serine-rich 14 antibody
    • Sugp2 antibody
    • SUGP2_HUMAN antibody
    • SURP and G-patch domain-containing protein 2 antibody
    see all

Images

  • All lanes : Anti-SFRS14/SUGP2 antibody (ab229528) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 120 kDa



    5% SDS-PAGE

  • SFRS14/SUGP2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 µg ab229528. Western blot was performed from the immunoprecipitate using ab229528.

    Lane 1: HeLa whole cell extract (Input).

    Lane 2: Control IgG IP in HeLa whole cell extract.

    Lane 3: ab229528 IP in HeLa whole cell extract.

  • All lanes : Anti-SFRS14/SUGP2 antibody (ab229528) at 1/1000 dilution

    Lane 1 : Neuro-2a (Mouse neuroblastoma cell line) whole cell extracts
    Lane 2 : C2C12 (Mouse myoblast cell line) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 120 kDa



    5% SDS-PAGE

References

ab229528 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229528.
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