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Rabbit polyclonal to SFRS2IP
Predicted to work with:
Rabbit, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Bat
Synthetic peptide corresponding to a region between residue 1098 and the C-terminus (residue 1148) of human SFRS2IP (CAA72121.1)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
ab72425 was affinity purified using an epitope specific to SFRS2IP immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/2000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 129 kDa).
Use at 2-5 µg/mg of lysate.
SFRS2IP plays a role in pre-mRNA alternative splicing by regulating spliceosome assembly.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS2IP antibody (ab72425)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human metastatic lymph node tissue labelling SFRS2IP with ab72425 at 1/200 (1µg/ml). Detection: DAB.
Western blot - Anti-SFRS2IP antibody (ab72425)
All lanes :
Anti-SFRS2IP antibody (ab72425) at 0.04 µg/ml
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Predicted band size:
Observed band size:
200 kDa (
why is the actual band size different from the predicted?
Immunoprecipitation - Anti-SFRS2IP antibody (ab72425)
Detection of SFRS2IP by Western Blot of Immunprecipitate.
ab72425 at 0.1µg/ml staining SFRS2IP in HeLa whole cell lysate immunoprecipitated using ab72425 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"