Key features and details
- Rabbit polyclonal to SFRS2IP
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SFRS2IP antibody
See all SFRS2IP primary antibodies
DescriptionRabbit polyclonal to SFRS2IP
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Bat
Synthetic peptide corresponding to a region between residue 1098 and the C-terminus (residue 1148) of human SFRS2IP (CAA72121.1)
- HeLa whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab72425 was affinity purified using an epitope specific to SFRS2IP immobilized on solid support.
Our Abpromise guarantee covers the use of ab72425 in the following tested applications.
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 129 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceSFRS2IP plays a role in pre-mRNA alternative splicing by regulating spliceosome assembly.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human metastatic lymph node tissue labelling SFRS2IP with ab72425 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-SFRS2IP antibody (ab72425) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 129 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Detection of SFRS2IP by Western Blot of Immunprecipitate.
ab72425 at 0.1µg/ml staining SFRS2IP in HeLa whole cell lysate immunoprecipitated using ab72425 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab72425 has not yet been referenced specifically in any publications.