Key features and details
- Mouse polyclonal to SFRS7/SRSF7
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SFRS7/SRSF7 antibody
See all SFRS7/SRSF7 primary antibodies
DescriptionMouse polyclonal to SFRS7/SRSF7
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Full length protein corresponding to Human SFRS7/SRSF7. (NP_001026854, 1 a.a. ~ 238 a.a)
- SFRS7/SRSF7 transfected lysate
This product was previously labelled as SFRS7
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab72616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionRequired for pre-mRNA splicing. Can also modulate alternative splicing in vitro. Represses the splicing of MAPT/Tau exon 10.
Tissue specificityBrain, liver, kidney and lung.
Sequence similaritiesBelongs to the splicing factor SR family.
Contains 1 CCHC-type zinc finger.
Contains 1 RRM (RNA recognition motif) domain.
modificationsExtensively phosphorylated on serine residues in the RS domain.
- Information by UniProt
- 9G8 antibody
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Immunocytochemistry of HeLa cells staining SFRS7/SRSF7 using ab72616 at 10 μg/ml.
All lanes : Anti-SFRS7/SRSF7 antibody (ab72616) at 1/500 dilution
Lane 1 : SFRS7/SRSF7 transfected 293T cell line
Lane 2 : Non transfected 293T cell line
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
ab72616 has not yet been referenced specifically in any publications.