Anti-SFRS9 antibody (ab155484)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-SFRS9 antibody
See all SFRS9 primary antibodies -
DescriptionRabbit polyclonal to SFRS9
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Pig
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Immunogen
Recombinant fragment corresponding to a region within amino acids 52-199 of Human SFRS9 (Uniprot: Q13242).
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Positive control
- 293T, A431, H1299, MOLT4 and Raji whole cell lysates.
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General notesKeep as concentrated solution.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89% PBS, 10% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 26 kDa. |
Target
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FunctionPlays a role in constitutive splicing and can modulate the selection of alternative splice sites. Represses the splicing of MAPT/Tau exon 10.
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Tissue specificityExpressed at high levels in the heart, kidney, pancreas and placenta, and at lower levels in the brain, liver, lung and skeletal muscle.
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Sequence similaritiesBelongs to the splicing factor SR family.
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsExtensively phosphorylated on serine residues in the RS domain. -
Cellular localizationNucleus. Cellular stresses such as heat shock may induce localization to discrete nuclear bodies termed SAM68 nuclear bodies (SNBs), HAP bodies, or stress bodies. Numerous splicing factors including SRSF1/SFRS1/SF2, SRSF7/SFRS7, SAFB and KHDRBS1/SAM68 accumulate at these structures, which may participate in the post-transcriptional regulation of mRNAs in stressed cells.
- Information by UniProt
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Database links
- Entrez Gene: 8683 Human
- Entrez Gene: 108014 Mouse
- Entrez Gene: 100154637 Pig
- Omim: 601943 Human
- SwissProt: Q13242 Human
- SwissProt: Q9D0B0 Mouse
- Unigene: 706889 Human
- Unigene: 728777 Human
see all -
Alternative names
- arginine/serine-rich 9 antibody
- Pre mRNA splicing factor SRp30C antibody
- Pre-mRNA-splicing factor SRp30C antibody
see all
Images
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Western blot - Anti-SFRS9 antibody (ab155484)All lanes : Anti-SFRS9 antibody (ab155484) at 1/500 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lane 3 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa
12% SDS PAGE
Protocols
References
This product has been referenced in:
- Roundtree IA et al. YTHDC1 mediates nuclear export of N6-methyladenosine methylated mRNAs. Elife 6:N/A (2017). Read more (PubMed: 28984244) »
