Anti-SFRS9/SRSF9 antibody (ab155484)
Key features and details
- Rabbit polyclonal to SFRS9/SRSF9
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-SFRS9/SRSF9 antibody
See all SFRS9/SRSF9 primary antibodies -
Description
Rabbit polyclonal to SFRS9/SRSF9 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human SFRS9/SRSF9. The exact sequence is proprietary. Center region.
Database link: Q13242 -
Positive control
- WB: HEK-293T, MOLT-4 and Raji whole cell lysates. ICC/IF: HCT 116 cells.
-
General notes
Keep as concentrated solution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 26 kDa.
|
|
ICC/IF |
1/100 - 1/1000.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 26 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
-
Function
Plays a role in constitutive splicing and can modulate the selection of alternative splice sites. Represses the splicing of MAPT/Tau exon 10. -
Tissue specificity
Expressed at high levels in the heart, kidney, pancreas and placenta, and at lower levels in the brain, liver, lung and skeletal muscle. -
Sequence similarities
Belongs to the splicing factor SR family.
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsExtensively phosphorylated on serine residues in the RS domain. -
Cellular localization
Nucleus. Cellular stresses such as heat shock may induce localization to discrete nuclear bodies termed SAM68 nuclear bodies (SNBs), HAP bodies, or stress bodies. Numerous splicing factors including SRSF1/SFRS1/SF2, SRSF7/SFRS7, SAFB and KHDRBS1/SAM68 accumulate at these structures, which may participate in the post-transcriptional regulation of mRNAs in stressed cells. - Information by UniProt
-
Database links
- Entrez Gene: 8683 Human
- Omim: 601943 Human
- SwissProt: Q13242 Human
- Unigene: 706889 Human
- Unigene: 728777 Human
-
Alternative names
- arginine/serine-rich 9 antibody
- Pre mRNA splicing factor SRp30C antibody
- Pre-mRNA-splicing factor SRp30C antibody
see all
Images
-
4% PFA-fixed HCT 116 (Human colon carcinoma cell line) cells stained for SFRS9/SRSF9 (green) using ab155484 at 1/500 dilution in ICC/IF. Blue: Hoechst 33342.
-
All lanes : Anti-SFRS9/SRSF9 antibody (ab155484) at 1/500 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate
Lane 3 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa
12% SDS PAGE
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab155484 has been referenced in 1 publication.
- Roundtree IA et al. YTHDC1 mediates nuclear export of N6-methyladenosine methylated mRNAs. Elife 6:N/A (2017). PubMed: 28984244