Overview

  • Product name

    Anti-SFT antibody [EPR13000(B)]
    See all SFT primary antibodies
  • Description

    Rabbit monoclonal [EPR13000(B)] to SFT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human SFT aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P51668

  • Positive control

    • Jurkat and Raji cell lysates, Human skeletal muscle lysate, Human thryoid gland carcinoma and prostate tissues, Jurkat cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab176561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 17 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of CHIP, TRAF6 and TRIM63/MURF1. Ubiquitinates CHIP-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.
    • Tissue specificity

      Ubiquitous. Up-regulated in livers of iron-overloaded patients with hereditary hemochromatosis.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Belongs to the ubiquitin-conjugating enzyme family.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • E2(17)KB1 antibody
      • SFT antibody
      • Stimulator of Fe transport antibody
      • UB2D1_HUMAN antibody
      • UBC 4/5 antibody
      • UBC4/5 antibody
      • UBC4/5 homolog antibody
      • UBC4/5, S. cerevisiae, homolog of antibody
      • UBCH 5 antibody
      • UBCH 5A antibody
      • UbcH5 antibody
      • UBCH5A antibody
      • Ube2d1 antibody
      • Ubiquitin carrier protein antibody
      • Ubiquitin carrier protein D1 antibody
      • Ubiquitin conjugating enzyme E2 17 kDa 1 antibody
      • Ubiquitin conjugating enzyme E2 D1 antibody
      • Ubiquitin conjugating enzyme E2D 1 (UBC4/5 homolog yeast) antibody
      • Ubiquitin conjugating enzyme E2D 1 antibody
      • Ubiquitin conjugating enzyme UBCH5A antibody
      • Ubiquitin protein ligase D1 antibody
      • Ubiquitin-conjugating enzyme E2 D1 antibody
      • Ubiquitin-conjugating enzyme E2(17)KB 1 antibody
      • Ubiquitin-conjugating enzyme E2-17 kDa 1 antibody
      • Ubiquitin-protein ligase D1 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: SFT knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab176561 observed at 18 kDa. Red - loading control, ab181602, observed at 37 kDa. ab176561 was shown to recognize SFT when SFT knockout samples were used, along with additional cross-reactive bands. Wild-type and SFT knockout samples were subjected to SDS-PAGE. ab176561 and ab181602 (loading control to GAPDH) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-SFT antibody [EPR13000(B)] (ab176561) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : Human skeletal muscle lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 17 kDa

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human prostate tissue labeling SFT with ab176561 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human thyroid gland carcinoma tissue labeling SFT with ab176561 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow cytometry analysis of permeabilized Jurkat cells labeling SFT (red) with ab176561 at a 1/10 dilution, or negative control rabbit IgG (green)

    • Western blot analysis on immunoprecipitation pellet from Human skeletal muscle lysate labeling SFT with ab176561 at 1/10 dilution.

    References

    This product has been referenced in:

    • Shi Y  et al. Ube2D3 and Ube2N are essential for RIG-I-mediated MAVS aggregation in antiviral innate immunity. Nat Commun 8:15138 (2017). WB ; Human . Read more (PubMed: 28469175) »
    • Liu XJ  et al. Human cytomegalovirus IE1 downregulates Hes1 in neural progenitor cells as a potential E3 ubiquitin ligase. PLoS Pathog 13:e1006542 (2017). Read more (PubMed: 28750047) »
    See all 2 Publications for this product

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