Overview

  • Product name

    Anti-SFT antibody [EPR13000(B)] - BSA and Azide free
    See all SFT primary antibodies
  • Description

    Rabbit monoclonal [EPR13000(B)] to SFT - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IP, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SFT aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P51668

  • General notes

    Ab240192 is the carrier-free version of ab176561. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240192 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of CHIP, TRAF6 and TRIM63/MURF1. Ubiquitinates CHIP-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.
    • Tissue specificity

      Ubiquitous. Up-regulated in livers of iron-overloaded patients with hereditary hemochromatosis.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Belongs to the ubiquitin-conjugating enzyme family.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • E2(17)KB1 antibody
      • SFT antibody
      • Stimulator of Fe transport antibody
      • UB2D1_HUMAN antibody
      • UBC 4/5 antibody
      • UBC4/5 antibody
      • UBC4/5 homolog antibody
      • UBC4/5, S. cerevisiae, homolog of antibody
      • UBCH 5 antibody
      • UBCH 5A antibody
      • UbcH5 antibody
      • UBCH5A antibody
      • Ube2d1 antibody
      • Ubiquitin carrier protein antibody
      • Ubiquitin carrier protein D1 antibody
      • Ubiquitin conjugating enzyme E2 17 kDa 1 antibody
      • Ubiquitin conjugating enzyme E2 D1 antibody
      • Ubiquitin conjugating enzyme E2D 1 (UBC4/5 homolog yeast) antibody
      • Ubiquitin conjugating enzyme E2D 1 antibody
      • Ubiquitin conjugating enzyme UBCH5A antibody
      • Ubiquitin protein ligase D1 antibody
      • Ubiquitin-conjugating enzyme E2 D1 antibody
      • Ubiquitin-conjugating enzyme E2(17)KB 1 antibody
      • Ubiquitin-conjugating enzyme E2-17 kDa 1 antibody
      • Ubiquitin-protein ligase D1 antibody
      see all

    Images

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human prostate tissue labeling SFT with ab176561 at a 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176561).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human thyroid gland carcinoma tissue labeling SFT with ab176561 at a 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176561).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow cytometry analysis of permeabilized Jurkat cells labeling SFT (red) with ab176561 at a 1/10 dilution, or negative control rabbit IgG (green)

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176561).

    • Western blot analysis on immunoprecipitation pellet from Human skeletal muscle lysate labeling SFT with ab176561 at 1/10 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176561).

    References

    ab240192 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab240192.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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