Overview

  • Product name

    Anti-SFT antibody [EPR13000(B)] (HRP)
    See all SFT primary antibodies
  • Description

    Rabbit monoclonal [EPR13000(B)] to SFT (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SFT aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P51668

  • Positive control

    • Jurkat, HeLa, Human skeletal muscle, HAP1 WT (shows pos.), SFT KO HAP1 (shows neg.)
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab215343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).

Target

  • Function

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of CHIP, TRAF6 and TRIM63/MURF1. Ubiquitinates CHIP-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.
  • Tissue specificity

    Ubiquitous. Up-regulated in livers of iron-overloaded patients with hereditary hemochromatosis.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • E2(17)KB1 antibody
    • SFT antibody
    • Stimulator of Fe transport antibody
    • UB2D1_HUMAN antibody
    • UBC 4/5 antibody
    • UBC4/5 antibody
    • UBC4/5 homolog antibody
    • UBC4/5, S. cerevisiae, homolog of antibody
    • UBCH 5 antibody
    • UBCH 5A antibody
    • UbcH5 antibody
    • UBCH5A antibody
    • Ube2d1 antibody
    • Ubiquitin carrier protein antibody
    • Ubiquitin carrier protein D1 antibody
    • Ubiquitin conjugating enzyme E2 17 kDa 1 antibody
    • Ubiquitin conjugating enzyme E2 D1 antibody
    • Ubiquitin conjugating enzyme E2D 1 (UBC4/5 homolog yeast) antibody
    • Ubiquitin conjugating enzyme E2D 1 antibody
    • Ubiquitin conjugating enzyme UBCH5A antibody
    • Ubiquitin protein ligase D1 antibody
    • Ubiquitin-conjugating enzyme E2 D1 antibody
    • Ubiquitin-conjugating enzyme E2(17)KB 1 antibody
    • Ubiquitin-conjugating enzyme E2-17 kDa 1 antibody
    • Ubiquitin-protein ligase D1 antibody
    see all

Images

  • All lanes : Anti-SFT antibody [EPR13000(B)] (HRP) (ab215343) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : SFT knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 17 kDa
    Observed band size: 17 kDa


    Exposure time: 30 seconds


    ab215343 was shown to recognize SFT in wild-type HAP1 cells as signal was lost at the expected MW in SFT knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SFT knockout samples were subjected to SDS-PAGE. Ab215343 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

  • All lanes : Anti-SFT antibody [EPR13000(B)] (HRP) (ab215343) at 1/5000 dilution

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
    Lane 3 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue at 10 µg
    Lane 4 : HAP1 WT at 20 µg
    Lane 5 : SFT KO HAP1 at 20 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 17 kDa
    Observed band size: 17 kDa


    Exposure time: 4 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab215343 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab215343 has not yet been referenced specifically in any publications.

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