Recombinant HRP Anti-SFT antibody [EPR13000(B)] (ab215343)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR13000(B)] to SFT
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-SFT antibody [EPR13000(B)]
See all SFT primary antibodies -
Description
HRP Rabbit monoclonal [EPR13000(B)] to SFT -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat, HeLa, Human skeletal muscle, HAP1 WT (shows pos.), SFT KO HAP1 (shows neg.)
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13000(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
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Notes |
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WB
1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). |
Target
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Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of CHIP, TRAF6 and TRIM63/MURF1. Ubiquitinates CHIP-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4. -
Tissue specificity
Ubiquitous. Up-regulated in livers of iron-overloaded patients with hereditary hemochromatosis. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7321 Human
- Omim: 602961 Human
- SwissProt: P51668 Human
- Unigene: 129683 Human
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Alternative names
- E2(17)KB1 antibody
- SFT antibody
- Stimulator of Fe transport antibody
see all
Images
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All lanes : HRP Anti-SFT antibody [EPR13000(B)] (ab215343) at 1/5000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : SFT knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 30 secondsab215343 was shown to recognize SFT in wild-type HAP1 cells as signal was lost at the expected MW in SFT knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SFT knockout samples were subjected to SDS-PAGE. Ab215343 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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All lanes : HRP Anti-SFT antibody [EPR13000(B)] (ab215343) at 1/5000 dilution
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue at 10 µg
Lane 4 : HAP1 WT at 20 µg
Lane 5 : SFT KO HAP1 at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab215343 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab215343 has not yet been referenced specifically in any publications.