Overview

  • Product name

    Anti-SGF29 antibody - N-terminal
  • Description

    Rabbit polyclonal to SGF29 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken
  • Immunogen

    Recombinant fragment corresponding to Human SGF29 aa 1-72 (N terminal).
    Sequence:

    MALVSADSRIAELLTELHQLIKQTQEERSRSEHNLVNIQKTHERMQTENK ISPYYRTKLRGLYTTAKADAEA


    Database link: Q96ES7

  • Positive control

    • Human testis tissue, MCF7 cell line.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab204367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in transcriptional regulation, through association with histone acetyltransferase (HAT) SAGA-type complexes like the TFTC-HAT, ATAC or STAGA complexes. Specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preferrence for trimethylated form (H3K4me3). In the SAGA-type complexes, required to recruit complexes to H3K4me. May be involved in MYC-mediated oncogenic transformation.
  • Sequence similarities

    Belongs to the SGF29 family.
    Contains 1 SGF29 C-terminal domain.
  • Domain

    The SGF29 tudor-like domain mediates binding to methylated 'Lys-4' of histone H3 (H3K4me).
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CCDC101 antibody
    • Coiled coil domain containing 101 antibody
    • Coiled coil domain containing protein 101 antibody
    • Coiled-coil domain-containing protein 101 antibody
    • FLJ32446 antibody
    • OTTMUSP00000030606 antibody
    • SAGA associated factor 29 homolog antibody
    • SAGA-associated factor 29 homolog antibody
    • SGF29_HUMAN antibody
    • STAF36 antibody
    see all

Images

  • Immunofluorescence analysis of MCF7 cells labeling SGF29 with ab204367 at 4 µg/ml.

     

  • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling SGF29 with ab204367 at 1/200 dilution.

References

ab204367 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab204367.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up