RecombinantRabMAb

Recombinant Anti-SGK1 antibody [Y238] (ab32374)

Submit a review Q&A (1)References (11)
Western blot - Anti-SGK1 antibody [Y238] (ab32374)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGK1 antibody [Y238] (ab32374)
  • Flow Cytometry - Anti-SGK1 antibody [Y238] (ab32374)
  • Anti-SGK1 antibody [Y238] (ab32374)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y238] to SGK1
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SGK1 antibody [Y238]
    See all SGK1 primary antibodies

  • Description

    Rabbit monoclonal [Y238] to SGK1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human SGK1 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: A431 and NIH3T3 cell lysates. IHC-P: Human kidney carcinoma.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab32374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 57 kDa (predicted molecular weight: 49 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC.

    • Target

    • Function

      Protein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, KCNA3/Kv1.3 or EAAT1. Isoform 2 exhibited a greater effect on cell plasma membrane expression of ENaC and Na(+) transport than isoform 1.

    • Tissue specificity

      Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.

    • Sequence similarities

      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 protein kinase domain.

    • Domain

      Isoform 2 subcellular localization at the plasma membrane is mediated by the sequences within the first 120 amino acids.

    • Post-translational
      modifications

      Regulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1.
      Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability. Isoform 2 resistance to proteasomal degradation is mediated by the sequences within the first 120-amino acid.

    • Cellular localization

      Cell membrane and Cytoplasm. Nucleus. Endoplasmic reticulum. Nuclear, upon phosphorylation.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • OTTHUMP00000017247 antibody
      • Serine/threonine protein kinase SGK antibody
      • Serine/threonine protein kinase Sgk1 antibody
      • Serine/threonine-protein kinase Sgk1 antibody
      • Serum and glucocorticoid regulated kinase antibody
      • Serum/glucocorticoid regulated kinase 1 antibody
      • Serum/glucocorticoid regulated kinase antibody
      • Serum/glucocorticoid-regulated kinase 1 antibody
      • SGK 1 antibody
      • SGK antibody
      • SGK1 antibody
      • Sgk1 variant i3 antibody
      • SGK1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-SGK1 antibody [Y238] (ab32374)
      Western blot - Anti-SGK1 antibody [Y238] (ab32374)
      All lanes : Anti-SGK1 antibody [Y238] (ab32374) at 1/500 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : NIH3T3 cell lysate

      Predicted band size: 49 kDa
      Observed band size: 57 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGK1 antibody [Y238] (ab32374)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGK1 antibody [Y238] (ab32374)

      Ab32374, at a 1/50 dilution, staining SGK1 in paraffin embedded human kidney carcinoma tissue sections by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-SGK1 antibody [Y238] (ab32374)
      Flow Cytometry - Anti-SGK1 antibody [Y238] (ab32374)
      Overlay histogram showing HEK293 cells stained with ab32374 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32374, 1/100) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Anti-SGK1 antibody [Y238] (ab32374)
      Anti-SGK1 antibody [Y238] (ab32374)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (11)

    Publishing research using ab32374? Please let us know so that we can cite the reference in this datasheet.

    ab32374 has been referenced in 11 publications.

    • Xiao L  et al. Spinal Serum- and Glucocorticoid-Regulated Kinase 1 (SGK1) Signaling Contributes to Morphine-Induced Analgesic Tolerance in Rats. Neuroscience 413:206-218 (2019). PubMed: 31220544
    • Pi H  et al. AKT inhibition-mediated dephosphorylation of TFE3 promotes overactive autophagy independent of MTORC1 in cadmium-exposed bone mesenchymal stem cells. Autophagy 15:565-582 (2019). PubMed: 30324847
    • Li J  et al. The mTOR kinase inhibitor everolimus synergistically enhances the anti-tumor effect of the Bruton's tyrosine kinase (BTK) inhibitor PLS-123 on Mantle cell lymphoma. Int J Cancer 142:202-213 (2018). PubMed: 28905990
    • Tang Z  et al. Serum and glucocorticoid-regulated kinase 1 (SGK1) is a predictor of poor prognosis in non-small cell lung cancer, and its dynamic pattern following treatment with SGK1 inhibitor and ?-ray irradiation was elucidated. Oncol Rep 39:1505-1515 (2018). PubMed: 29328462
    • Sumida T  et al. Activated ß-catenin in Foxp3+ regulatory T cells links inflammatory environments to autoimmunity. Nat Immunol 19:1391-1402 (2018). PubMed: 30374130
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Your data sheet for the anti-SGK1 antibody # ab32374 describes the immunogen as being "a synthetic peptide corresponding to residues of human SGK1." Can you tell me which residues, or if that is proprietary, could you tell me if the immunogen is at the C-terminus as your other SGK1 antibody is? I'm trying to find an SGK antibody that will reliably recognize SGK1 in killifish. At the moment, I'm using an antibody from another company using the C-term end of human SGK as the immunogen. In theory, it should recongize the killifish SGK but doesn't give good bands. I'm trying to find another antibody to use in its place. Thank you for your help.

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    Answer

    Thank you for your enquiry. The homology is only 72%, 13/18. The immunogen sequence was taken from about 50 AA in from the N-terminus. I usually advise that anything over 85% homology is likely to cross-react. 72% is a little low, but still may work. Please do not hesitate to contact us if you need anything further.

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