Anti-SGK3 antibody (ab153981)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-SGK3 antibody
See all SGK3 primary antibodies -
Description
Rabbit polyclonal to SGK3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-287 of Human SGK3 (Q96BR1).
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Positive control
- HepG2 whole cell lysate (ab7900). HeLa cells. Human adrenal gland tumor tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab153981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 57 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Involved in the activation of potassium channels. Mediates cell IL-3-dependent survival signals. -
Tissue specificity
Expressed in most tissues with highest levels in pancreas, kidney liver, heart and brain and lower levels in lung, placenta and skeletal muscle. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 PX (phox homology) domain. -
Cellular localization
Cytoplasmic vesicle. Early endosome. Localized in vesicle-like structures and in the early endosome. - Information by UniProt
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Database links
- Entrez Gene: 100533105 Human
- Entrez Gene: 23678 Human
- Omim: 607591 Human
- SwissProt: Q96BR1 Human
- Unigene: 613417 Human
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Alternative names
- CISK antibody
- Cytokine independent survival kinase antibody
- DKFZp781N0293 antibody
see all
Images
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Western blot - Anti-SGK3 antibody (ab153981)Anti-SGK3 antibody (ab153981) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 57 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-SGK3 antibody (ab153981)Immunofluorescence analysis of methanol-fixed HeLa cells, labeling SGK3 with ab153981 at 1/200 dilution. Lower panel is costained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGK3 antibody (ab153981)Immunohistochemical analysis of paraffin-embedded Human adrenal gland tumor tissue labeling SGK3 with ab153981 at 1/500 dilution.
Protocols
References
This product has been referenced in:
- Chu W et al. Lovastatin exerts protective effects on endothelial cells via upregulation of PTK2B. Exp Ther Med 12:1741-1749 (2016). Read more (PubMed: 27602089) »
