Anti-SGK3 antibody (ab153981)
Key features and details
- Rabbit polyclonal to SGK3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SGK3 antibody
See all SGK3 primary antibodies -
Description
Rabbit polyclonal to SGK3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-287 of Human SGK3 (Q96BR1).
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Positive control
- HepG2 whole cell lysate (ab7900). HeLa cells. Human adrenal gland tumor tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab153981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 57 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Involved in the activation of potassium channels. Mediates cell IL-3-dependent survival signals. -
Tissue specificity
Expressed in most tissues with highest levels in pancreas, kidney liver, heart and brain and lower levels in lung, placenta and skeletal muscle. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 PX (phox homology) domain. -
Cellular localization
Cytoplasmic vesicle. Early endosome. Localized in vesicle-like structures and in the early endosome. - Information by UniProt
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Database links
- Entrez Gene: 100533105 Human
- Entrez Gene: 23678 Human
- Omim: 607591 Human
- SwissProt: Q96BR1 Human
- Unigene: 613417 Human
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Alternative names
- CISK antibody
- Cytokine independent survival kinase antibody
- DKFZp781N0293 antibody
see all
Images
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Western blot - Anti-SGK3 antibody (ab153981)Anti-SGK3 antibody (ab153981) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 57 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-SGK3 antibody (ab153981)Immunofluorescence analysis of methanol-fixed HeLa cells, labeling SGK3 with ab153981 at 1/200 dilution. Lower panel is costained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGK3 antibody (ab153981)Immunohistochemical analysis of paraffin-embedded Human adrenal gland tumor tissue labeling SGK3 with ab153981 at 1/500 dilution.
Protocols
References (1)
ab153981 has been referenced in 1 publication.
- Chu W et al. Lovastatin exerts protective effects on endothelial cells via upregulation of PTK2B. Exp Ther Med 12:1741-1749 (2016). PubMed: 27602089
