Anti-SGLT1 antibody (ab14685)
Key features and details
- Rabbit polyclonal to SGLT1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SGLT1 antibody
See all SGLT1 primary antibodies -
Description
Rabbit polyclonal to SGLT1 -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 75% homology with SGLT5. Cross-reactivity with this protein has not been confirmed experimentally.
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Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human SGLT1 aa 600-700.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: Tris glycine, 0.5% BSA, HEPES, 30% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use a concentration of 2 µg/ml.
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Notes |
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IHC-P
Use a concentration of 2 µg/ml. |
Target
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Function
Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules. -
Tissue specificity
Expressed mainly in intestine and kidney. -
Involvement in disease
Congenital glucose/galactose malabsorption -
Sequence similarities
Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. -
Post-translational
modificationsN-glycosylation is not necessary for the cotransporter function. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6523 Human
- Omim: 182380 Human
- SwissProt: P13866 Human
- Unigene: 1964 Human
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Alternative names
- D22S675 antibody
- High affinity sodium glucose cotransporter 1 antibody
- High affinity sodium glucose cotransporter antibody
see all
Images
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ab14685 (2µg/ml) staining SGLT1 in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the apical epithelium cells of the villi.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Datasheets and documents
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SDS download
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Datasheet download
References (22)
ab14685 has been referenced in 22 publications.
- Du J et al. The expression and survival significance of sodium glucose transporters in pancreatic cancer. BMC Cancer 22:116 (2022). PubMed: 35090421
- Park SH et al. Angiotensin II-induced upregulation of SGLT1 and 2 contributes to human microparticle-stimulated endothelial senescence and dysfunction: protective effect of gliflozins. Cardiovasc Diabetol 20:65 (2021). PubMed: 33726768
- Ranea-Robles P et al. Peroxisomal L-bifunctional protein (EHHADH) deficiency causes male-specific kidney hypertrophy and proximal tubular injury in mice. Kidney360 2:1441-1454 (2021). PubMed: 34651140
- Chiba Y et al. Sodium/glucose cotransporter 2 is expressed in choroid plexus epithelial cells and ependymal cells in human and mouse brains. Neuropathology N/A:N/A (2020). PubMed: 32488949
- Chiba Y et al. Glucose, Fructose, and Urate Transporters in the Choroid Plexus Epithelium. Int J Mol Sci 21:N/A (2020). PubMed: 33008107