Overview

  • Product name

  • Description

    Rabbit polyclonal to SGLT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Dot blot, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 603-623 of Human SGLT1

Properties

Applications

Our Abpromise guarantee covers the use of ab14685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 55-62 kDa.
Dot blot 1/4000.
ELISA 1/4000.

Target

  • Function

    Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.
  • Tissue specificity

    Expressed mainly in intestine and kidney.
  • Involvement in disease

    Congenital glucose/galactose malabsorption
  • Sequence similarities

    Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
  • Post-translational
    modifications

    N-glycosylation is not necessary for the cotransporter function.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • D22S675 antibody
    • High affinity sodium glucose cotransporter 1 antibody
    • High affinity sodium glucose cotransporter antibody
    • High affinity sodium-glucose cotransporter antibody
    • Human Na+/glucose cotransporter 1 antibody
    • Na(+)/glucose cotransporter 1 antibody
    • Na+/glucose cotransporter 1 antibody
    • NAGT antibody
    • SC5A1_HUMAN antibody
    • SGLT 1 antibody
    • SGLT1 antibody
    • SLC5A1 antibody
    • Sodium glucose cotransporter 1 antibody
    • Sodium/glucose cotransporter 1 antibody
    • Solute carrier family 5 (sodium/glucose cotransporter) member 1 antibody
    • Solute carrier family 5 member 1 antibody
    see all

Images

  • ab14685 (2µg/ml) staining SGLT1 in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the apical epithelium cells of the villi.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References

This product has been referenced in:

  • Suga T  et al. SGLT1 in pancreatic a cells regulates glucagon secretion in mice, possibly explaining the distinct effects of SGLT2 inhibitors on plasma glucagon levels. Mol Metab 19:1-12 (2019). Read more (PubMed: 30416006) »
  • Ono E  et al. Sweat glucose and GLUT2 expression in atopic dermatitis: Implication for clinical manifestation and treatment. PLoS One 13:e0195960 (2018). Read more (PubMed: 29677207) »
See all 13 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue (Kidney tissue)
Permeabilization
Yes - 0.3% Triton included in blocking buffer
Specification
Kidney tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Formalin

Abcam user community

Verified customer

Submitted Apr 23 2019

Question
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ***.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Answer

Thank you for your reply.


I have requested that a vial of ab14685 from a newer lot be added to your next order free of charge. You will receive confirmation shortly.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Read More

Answer

Thank you for contacting Abcam regarding ab14685.


I have reviewed the information provided by the customer and I agree that it is not performing as stated on the datasheet. I checked our stock and we do have a different lot available. Would the customer be interested in receiving a different lot or is a credit preferred?


I look forward to your reply so that I may assist you further. Please do not hesitate to contact us if you have any additional questions.

Read More

Answer

Thank you for your inquiry. I can confirm that rat SGLT1 has a predicted  molecular weight of ca. 73kDa. Molecular weights on gel are always only a rough estimate since the gel and buffer conditions can influence the resulting molecular weight of a protein dramatically. It is know in this case that also the percentage of the gel has an influence on the molecular weight of SGLT1. I hope i could reassure your customer. Please do not hesitate to contact me again with any further questions in this matter.  

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Bladder cancer cells)
Loading amount
60 µg
Specification
Human Bladder cancer cells
Treatment
Betulinic acid, curcumin, Gefitinib for 48 hrs
Gel Running Conditions
Reduced Denaturing (7%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 16 2010

Answer

Thank you for your patience. For ab14685 the immunogen used was a synthetic peptide, corresponding to amino acids 603-623 of Human SGLT1. For ab14686 the immunogen used was a synthetic peptide, corresponding to amino acids 402-422 of Human SGLT1. Please contact us again if you have any additional questions.

Read More

Answer

The synthetic peptide sequence was: "RRA YDL FCG LEQ HGA PKM TEE C". The peptide was cyclize by disulfide bond between Cys 8 and Cys 22.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up