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Dear Sir/ Madam, Sorry to disturb. Our customer raises an inquiry between ab14685 and ab14686 (both Anti-SGLT1 antibody). She would like to conduct WB with rat sample, and these two products meet her need. The predicted protein size is around 73kDa in the human as well as the rat species. But she is confused about the detected molecular weight in the datasheet of ab14685: “Detects a band of approximately 55-62 kDa”. She therefore would like to confirm the actual molecular weight in Rat sample when using these 2 antibodies. Would you please help her deal with this issue? Thank you very much. Beat regards,
Asked on Nov 10 2011
Thank you for your inquiry. I can confirm that rat SGLT1 has a predicted molecular weight of ca. 73kDa. Molecular weights on gel are always only a rough estimate since the gel and buffer conditions can influence the resulting molecular weight of a protein dramatically. It is know in this case that also the percentage of the gel has an influence on the molecular weight of SGLT1. I hope i could reassure your customer. Please do not hesitate to contact me again with any further questions in this matter.
Answered on Nov 10 2011