Overview

  • Product name
  • Description
    Rabbit polyclonal to SGLT1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Dot blot, ELISA, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic peptide, corresponding to amino acids 402-422 of Human SGLT1

  • Positive control
  • General notes

    A blocking peptide for this product is available as ab190911.

Properties

Applications

Our Abpromise guarantee covers the use of ab14686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 73 kDa).Can be blocked with SGLT1 peptide (ab190911).
Dot blot 1/4000.
ELISA 1/4000.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.
  • Tissue specificity
    Expressed mainly in intestine and kidney.
  • Involvement in disease
    Congenital glucose/galactose malabsorption
  • Sequence similarities
    Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
  • Post-translational
    modifications
    N-glycosylation is not necessary for the cotransporter function.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • D22S675 antibody
    • High affinity sodium glucose cotransporter 1 antibody
    • High affinity sodium glucose cotransporter antibody
    • High affinity sodium-glucose cotransporter antibody
    • Human Na+/glucose cotransporter 1 antibody
    • Na(+)/glucose cotransporter 1 antibody
    • Na+/glucose cotransporter 1 antibody
    • NAGT antibody
    • SC5A1_HUMAN antibody
    • SGLT 1 antibody
    • SGLT1 antibody
    • SLC5A1 antibody
    • Sodium glucose cotransporter 1 antibody
    • Sodium/glucose cotransporter 1 antibody
    • Solute carrier family 5 (sodium/glucose cotransporter) member 1 antibody
    • Solute carrier family 5 member 1 antibody
    see all

Images

  • Immunohistochemistry - Anti-SGLT1 antibody (ab14686). Mouse Intestine - SGLT1, Primary Antibody: SGLT-112AP; 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain.

  • Immunohistochemistry - Anti-SGLT1 antibody (ab14686). Baboon Small Intestine - SGLT1, Primary Antibody: SGLT-101AP; 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification. FFPE section

  • Immunohistochemistry - Anti-SGLT1 antibody (ab14686). Baboon Small Intestine - SGLT1, Primary Antibody: SGLT-101AP; 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 10X magnification. FFPE section

  • Anti-SGLT1 antibody (ab14686) at 1/500 dilution
    Predicted band size: 73 kDa
    Observed band size: 72 kDa
    why is the actual band size different from the predicted?



    The positive control for Sglt1 was purified rat jujenal brush border membranes solobilized in Solo buffer at approximately 1.5 mg per ml. A 65 ul sample was applied on 8.5% gel, the approximate MWt of rat Sglt varies between MW of 70-74kda depending upon the concentration of acrylamide in the gel. The blot was developed with colorimetric development using alkaline phosphatase conjugate.
  • Immunohistochemical analysis of formalin fixed, paraffin embedded porcine duodenum tissue from fasted animals. SGLT1 is stained with ab14686 at 1/50 diluton.

References

This product has been referenced in:
  • Klinger S  et al. Degree of SGLT1 phosphorylation is associated with but does not determine segment-specific glucose transport features in the porcine small intestines. Physiol Rep 6:N/A (2018). Read more (PubMed: 29333720) »
  • Shao Y  et al. EGFR-TKI resistance and MAP17 are associated with cancer stem cell like properties. Oncol Lett 15:6655-6665 (2018). Read more (PubMed: 29616128) »
See all 21 Publications for this product

Customer reviews and Q&As

1-10 of 12 Abreviews or Q&A

Application
Western blot
Sample
Human Tissue lysate - whole (Left ventricle)
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
50 µg
Specification
Left ventricle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Christopher Trummer

Verified customer

Submitted Oct 05 2018

Application
Western blot
Sample
Pig Tissue lysate - whole (Left ventricle)
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
50 µg
Specification
Left ventricle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Christopher Trummer

Verified customer

Submitted Oct 05 2018

Application
Western blot
Sample
Mouse Tissue lysate - whole (Ileum)
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
50 µg
Specification
Ileum
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Christopher Trummer

Verified customer

Submitted Oct 05 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Ileum)
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
50 µg
Specification
Ileum
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Christopher Trummer

Verified customer

Submitted Oct 04 2018

Application
Western blot
Sample
Guinea pig Tissue lysate - whole (Ileum)
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
50 µg
Specification
Ileum
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Christopher Trummer

Verified customer

Submitted Oct 04 2018

Question
Answer

Thank you for your enquiry. I am afraid that there is no further information regarding the positive control that what is already stated on the datasheet. "The positive control for Sglt1 was purified rat jujenal brush border membranes solobilized in Solo buffer at approximately 1.5 mg per ml. A 65 ul sample was applied on 8.5% gel, the approximate MWt of rat Sglt varies between MW of 70-74kda depending upon the concentration of acrylamide in the gel." However, for your information the actual positive control used was product ab99447 SGLT1 protein. I hope this information will still be helpful.

Read More

Answer

Thank you for your inquiry. I can confirm that rat SGLT1 has a predicted  molecular weight of ca. 73kDa. Molecular weights on gel are always only a rough estimate since the gel and buffer conditions can influence the resulting molecular weight of a protein dramatically. It is know in this case that also the percentage of the gel has an influence on the molecular weight of SGLT1. I hope i could reassure your customer. Please do not hesitate to contact me again with any further questions in this matter.  

Read More

Answer

I am sorry to hear that you have been experiencing problems using this product in the application that you wish. It is known that more than 10 types of glucose transport carrier proteins designated as Glut 1-13 facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encodes at least 7 proteins exhibiting high degree of amino acid homology (45%-65%) all in the molecular weight range of 40-60 kDa. Please could you provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response. Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Specification
Kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6,0
Permeabilization
No
Blocking step
Peroxidase Blocking solution (Dako) as blocking agent for 5 minute(s) · Concentration: 3% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 06 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney cortex)
Specification
Kidney cortex
Fixative
Formalin
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Dako CSA II kit reagent as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 03 2008

1-10 of 12 Abreviews or Q&A

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