Anti-SGLT1 antibody (ab14686)

Reviews (9)Q&A (5)References (37)
Western blot - Anti-SGLT1 antibody (ab14686)

    Key features and details

    • Rabbit polyclonal to SGLT1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-SGLT1 antibody
      See all SGLT1 primary antibodies

    • Description

      Rabbit polyclonal to SGLT1

    • Host species

      Rabbit

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Synthetic peptide corresponding to Human SGLT1 aa 402-422. Synthetic peptide, corresponding to amino acids 402-422 of Human SGLT1
      Database link: P13866
      (Peptide available as ab190911)

    • Positive control

      • WB: Enterocytes, heart and skeletal muscle tissues

    • General notes

      A blocking peptide for this product is available as ab190911.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab14686 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB (6)
    1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 73 kDa).Can be blocked with SGLT1 peptide (ab190911).
    Notes
    WB
    1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 73 kDa).Can be blocked with SGLT1 peptide (ab190911).

    Target

    • Function

      Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.

    • Tissue specificity

      Expressed mainly in intestine and kidney.

    • Involvement in disease

      Congenital glucose/galactose malabsorption

    • Sequence similarities

      Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.

    • Post-translational
      modifications

      N-glycosylation is not necessary for the cotransporter function.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • D22S675 antibody
        • High affinity sodium glucose cotransporter 1 antibody
        • High affinity sodium glucose cotransporter antibody
        • High affinity sodium-glucose cotransporter antibody
        • Human Na+/glucose cotransporter 1 antibody
        • Na(+)/glucose cotransporter 1 antibody
        • Na+/glucose cotransporter 1 antibody
        • NAGT antibody
        • SC5A1_HUMAN antibody
        • SGLT 1 antibody
        • SGLT1 antibody
        • SLC5A1 antibody
        • Sodium glucose cotransporter 1 antibody
        • Sodium/glucose cotransporter 1 antibody
        • Solute carrier family 5 (sodium/glucose cotransporter) member 1 antibody
        • Solute carrier family 5 member 1 antibody
        see all

      Images

      • Western blot - Anti-SGLT1 antibody (ab14686)
        Western blot - Anti-SGLT1 antibody (ab14686)
        All lanes : Anti-SGLT1 antibody (ab14686) at 1/500 dilution

        Lane 1 : Enterocytes
        Lane 2 : Heart tissue
        Lane 3 : Skeletal muscle tissue

        Predicted band size: 73 kDa



        Tissue extracts were made in Solobuffer with protease inhibitors. The samples were solubilized in SDS-PAGE sample buffer and reduced with 2.5% BME.
        Total 40-50ug protein was applied per lane on 10% SDS PAGE gels.
        The proteins were transferred to nitrocellulose F and blocked with 5% milk and blotted with 1:500 diluted ab14686. 

        The image was obtained with high sensitivity ECL assay and Image was captured with a CCD cooled camera for 30 sec.

      References (37)

      Publishing research using ab14686? Please let us know so that we can cite the reference in this datasheet.

      ab14686 has been referenced in 37 publications.

      • Blanco AM  et al. FGF21 Mimics a Fasting-Induced Metabolic State and Increases Appetite in Zebrafish. Sci Rep 10:6993 (2020). PubMed: 32332781
      • Koyani CN  et al. Empagliflozin protects heart from inflammation and energy depletion via AMPK activation. Pharmacol Res 158:104870 (2020). PubMed: 32434052
      • Wang J  et al. The positive feedback between ACSL4 expression and O-GlcNAcylation contributes to the growth and survival of hepatocellular carcinoma. Aging (Albany NY) 12:7786-7800 (2020). PubMed: 32357142
      • Zhang H  et al. Molecular Mechanisms Underlying the Absorption of Aglycone and Glycosidic Flavonoids in a Caco-2 BBe1 Cell Model. ACS Omega 5:10782-10793 (2020). PubMed: 32455198
      • Sunilkumar S & Ford SM Elevated glucose concentration in culture media decreases membrane trafficking of SGLT2 in LLC-PK1 cells via a cAMP/PKA-dependent pathway. Am J Physiol Cell Physiol 316:C913-C924 (2019). PubMed: 30943059
      View all Publications for this product

      Customer reviews and Q&As

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      1-10 of 14 Abreviews or Q&A

      Immunohistochemistry (Frozen sections) abreview for Anti-SGLT1 antibody

       Inconclusive
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (Large intestine(ascending colon) and small intesti)
      Permeabilization
      Yes - 0.5%TritonX100
      Specification
      Large intestine(ascending colon) and small intesti
      Blocking step
      Donkey Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 13 2020

      Western blot abreview for Anti-SGLT1 antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Cortex)
      Gel Running Conditions
      Reduced Non-Denaturing (Native)
      Loading amount
      50 µg
      Specification
      Cortex
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jun 19 2019

      Western blot abreview for Anti-SGLT1 antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Tissue lysate - whole (Left ventricle)
      Gel Running Conditions
      Reduced Non-Denaturing (Native)
      Loading amount
      50 µg
      Specification
      Left ventricle
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Christopher Trummer

      Verified customer

      Submitted Oct 05 2018

      Western blot abreview for Anti-SGLT1 antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Pig Tissue lysate - whole (Left ventricle)
      Gel Running Conditions
      Reduced Non-Denaturing (Native)
      Loading amount
      50 µg
      Specification
      Left ventricle
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Christopher Trummer

      Verified customer

      Submitted Oct 05 2018

      Western blot abreview for Anti-SGLT1 antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Ileum)
      Gel Running Conditions
      Reduced Non-Denaturing (Native)
      Loading amount
      50 µg
      Specification
      Ileum
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Christopher Trummer

      Verified customer

      Submitted Oct 05 2018

      Western blot abreview for Anti-SGLT1 antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Tissue lysate - whole (Ileum)
      Gel Running Conditions
      Reduced Non-Denaturing (Native)
      Loading amount
      50 µg
      Specification
      Ileum
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Christopher Trummer

      Verified customer

      Submitted Oct 04 2018

      Western blot abreview for Anti-SGLT1 antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Guinea pig Tissue lysate - whole (Ileum)
      Gel Running Conditions
      Reduced Non-Denaturing (Native)
      Loading amount
      50 µg
      Specification
      Ileum
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Christopher Trummer

      Verified customer

      Submitted Oct 04 2018

      Question

      How was the positive control prepared?

      Read More
      Answer

      Thank you for your enquiry. I am afraid that there is no further information regarding the positive control that what is already stated on the datasheet. "The positive control for Sglt1 was purified rat jujenal brush border membranes solobilized in Solo buffer at approximately 1.5 mg per ml. A 65 ul sample was applied on 8.5% gel, the approximate MWt of rat Sglt varies between MW of 70-74kda depending upon the concentration of acrylamide in the gel." However, for your information the actual positive control used was product ab99447 SGLT1 protein. I hope this information will still be helpful.

      Read More

      Question

      Dear Sir/ Madam, Sorry to disturb. Our customer raises an inquiry between ab14685 and ab14686 (both Anti-SGLT1 antibody). She would like to conduct WB with rat sample, and these two products meet her need. The predicted protein size is around 73kDa in the human as well as the rat species. But she is confused about the detected molecular weight in the datasheet of ab14685: “Detects a band of approximately 55-62 kDa”. She therefore would like to confirm the actual molecular weight in Rat sample when using these 2 antibodies. Would you please help her deal with this issue? Thank you very much. Beat regards,

      Read More
      Answer

      Thank you for your inquiry. I can confirm that rat SGLT1 has a predicted  molecular weight of ca. 73kDa. Molecular weights on gel are always only a rough estimate since the gel and buffer conditions can influence the resulting molecular weight of a protein dramatically. It is know in this case that also the percentage of the gel has an influence on the molecular weight of SGLT1. I hope i could reassure your customer. Please do not hesitate to contact me again with any further questions in this matter.  

      Read More

      Question

      Hello, I´m trying to perform a Western Blot for Human SGLT1(using anti-SGLT1 ab14686), but I have problems with the blotting. I think there´s a problem with the cellular lysis. I already tried to use different lysis buffer (e.g. RIPA, Rotiload) and different temperatures, but either the samples remain in the pockets of the gel or I detect a wrong size (aroud 40 kDa). Can you please write me the protocol you used or give me any suggestions? Thank you very much. Kind Regards,  

      Read More
      Answer

      I am sorry to hear that you have been experiencing problems using this product in the application that you wish. It is known that more than 10 types of glucose transport carrier proteins designated as Glut 1-13 facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encodes at least 7 proteins exhibiting high degree of amino acid homology (45%-65%) all in the molecular weight range of 40-60 kDa. Please could you provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response. Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon.

      Read More

      1-10 of 14 Abreviews or Q&A

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