Anti-SGLT1 antibody (ab14686)

Thank you for your enquiry. I am afraid that there is no further information regarding the positive control that what is already stated on the datasheet. "The positive control for Sglt1 was purified rat jujenal brush border membranes solobilized in Solo buffer at approximately 1.5 mg per ml. A 65 ul sample was applied on 8.5% gel, the approximate MWt of rat Sglt varies between MW of 70-74kda depending upon the concentration of acrylamide in the gel." However, for your information the actual positive control used was product ab99447 SGLT1 protein. I hope this information will still be helpful.

Thank you for your inquiry. I can confirm that rat SGLT1 has a predicted  molecular weight of ca. 73kDa. Molecular weights on gel are always only a rough estimate since the gel and buffer conditions can influence the resulting molecular weight of a protein dramatically. It is know in this case that also the percentage of the gel has an influence on the molecular weight of SGLT1. I hope i could reassure your customer. Please do not hesitate to contact me again with any further questions in this matter.  

I am sorry to hear that you have been experiencing problems using this product in the application that you wish. It is known that more than 10 types of glucose transport carrier proteins designated as Glut 1-13 facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encodes at least 7 proteins exhibiting high degree of amino acid homology (45%-65%) all in the molecular weight range of 40-60 kDa. Please could you provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response. Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon.