Anti-SGLT1 antibody (ab14686)
- Datasheet
- References (24)
- Protocols
Overview
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Product nameAnti-SGLT1 antibody
See all SGLT1 primary antibodies -
DescriptionRabbit polyclonal to SGLT1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, Dot blot, ELISA, IP, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human, Pig
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Immunogen
Synthetic peptide, corresponding to amino acids 402-422 of Human SGLT1
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Positive control
- WB: SGLT1 protein (ab99447).
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General notes
A blocking peptide for this product is available as ab190911.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium azide
Constituents: Tris glycine, 0.5% BSA, HEPES, 30% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified on antigen sepharose affinity column
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab14686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 73 kDa).Can be blocked with SGLT1 peptide (ab190911). | |
| Dot blot | 1/4000. | |
| ELISA | 1/4000. | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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FunctionActively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.
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Tissue specificityExpressed mainly in intestine and kidney.
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Involvement in diseaseCongenital glucose/galactose malabsorption
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Sequence similaritiesBelongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
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Post-translational
modificationsN-glycosylation is not necessary for the cotransporter function. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 6523 Human
- Entrez Gene: 20537 Mouse
- Entrez Gene: 397113 Pig
- Entrez Gene: 25552 Rat
- Omim: 182380 Human
- SwissProt: P13866 Human
- SwissProt: Q8C3K6 Mouse
- SwissProt: P53790 Rat
see all -
Alternative names
- D22S675 antibody
- High affinity sodium glucose cotransporter 1 antibody
- High affinity sodium glucose cotransporter antibody
see all
Images
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Western blot - Anti-SGLT1 antibody (ab14686) -
Immunohistochemistry - Anti-SGLT1 antibody (ab14686)Immunohistochemistry - Anti-SGLT1 antibody (ab14686). Mouse Intestine - SGLT1, Primary Antibody: SGLT-112AP; 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody (ab14686)Immunohistochemistry - Anti-SGLT1 antibody (ab14686). Baboon Small Intestine - SGLT1, Primary Antibody: SGLT-101AP; 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification. FFPE section
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody (ab14686)Immunohistochemistry - Anti-SGLT1 antibody (ab14686). Baboon Small Intestine - SGLT1, Primary Antibody: SGLT-101AP; 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 10X magnification. FFPE section
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Western blot - Anti-SGLT1 antibody (ab14686)Anti-SGLT1 antibody (ab14686) at 1/500 dilution
Predicted band size: 73 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
The positive control for Sglt1 was purified rat jujenal brush border membranes solobilized in Solo buffer at approximately 1.5 mg per ml. A 65 ul sample was applied on 8.5% gel, the approximate MWt of rat Sglt varies between MW of 70-74kda depending upon the concentration of acrylamide in the gel. The blot was developed with colorimetric development using alkaline phosphatase conjugate. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT1 antibody (ab14686)Image from Asanuma-Date K et al., J Biol Chem. 2012 Jun 29;287(27):23104-18. Epub 2012 May 14.; Fig 1.; doi: 10.1074/jbc.M111.314658; June 29, 2012, The Journal of Biological Chemistry, 287, 23104-23118.Immunohistochemical analysis of formalin fixed, paraffin embedded porcine duodenum tissue from fasted animals. SGLT1 is stained with ab14686 at 1/50 diluton.
References
This product has been referenced in:
- Klinger S et al. Degree of SGLT1 phosphorylation is associated with but does not determine segment-specific glucose transport features in the porcine small intestines. Physiol Rep 6:N/A (2018). Read more (PubMed: 29333720) »
- Shao Y et al. EGFR-TKI resistance and MAP17 are associated with cancer stem cell like properties. Oncol Lett 15:6655-6665 (2018). Read more (PubMed: 29616128) »
