Anti-SGLT1 antibody (ab14686)
Key features and details
- Rabbit polyclonal to SGLT1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SGLT1 antibody
See all SGLT1 primary antibodies -
Description
Rabbit polyclonal to SGLT1 -
Host species
Rabbit -
Tested Applications & Species
Application Species WB Human -
Immunogen
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Positive control
- WB: Enterocytes, heart and skeletal muscle tissues
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General notes
A blocking peptide for this product is available as ab190911.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: Tris glycine, 0.5% BSA, HEPES, 30% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified on antigen sepharose affinity column -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Human
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Application | Abreviews | Notes |
---|---|---|
WB | (6) |
1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 73 kDa).Can be blocked with SGLT1 peptide (ab190911).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 73 kDa).Can be blocked with SGLT1 peptide (ab190911). |
Target
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Function
Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules. -
Tissue specificity
Expressed mainly in intestine and kidney. -
Involvement in disease
Congenital glucose/galactose malabsorption -
Sequence similarities
Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. -
Post-translational
modificationsN-glycosylation is not necessary for the cotransporter function. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6523 Human
- Omim: 182380 Human
- SwissProt: P13866 Human
- Unigene: 1964 Human
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Alternative names
- D22S675 antibody
- High affinity sodium glucose cotransporter 1 antibody
- High affinity sodium glucose cotransporter antibody
see all
Images
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All lanes : Anti-SGLT1 antibody (ab14686) at 1/500 dilution
Lane 1 : Enterocytes
Lane 2 : Heart tissue
Lane 3 : Skeletal muscle tissue
Predicted band size: 73 kDaTissue extracts were made in Solobuffer with protease inhibitors. The samples were solubilized in SDS-PAGE sample buffer and reduced with 2.5% BME.
Total 40-50ug protein was applied per lane on 10% SDS PAGE gels.
The proteins were transferred to nitrocellulose F and blocked with 5% milk and blotted with 1:500 diluted ab14686.The image was obtained with high sensitivity ECL assay and Image was captured with a CCD cooled camera for 30 sec.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (37)
ab14686 has been referenced in 37 publications.
- Blanco AM et al. FGF21 Mimics a Fasting-Induced Metabolic State and Increases Appetite in Zebrafish. Sci Rep 10:6993 (2020). PubMed: 32332781
- Koyani CN et al. Empagliflozin protects heart from inflammation and energy depletion via AMPK activation. Pharmacol Res 158:104870 (2020). PubMed: 32434052
- Wang J et al. The positive feedback between ACSL4 expression and O-GlcNAcylation contributes to the growth and survival of hepatocellular carcinoma. Aging (Albany NY) 12:7786-7800 (2020). PubMed: 32357142
- Zhang H et al. Molecular Mechanisms Underlying the Absorption of Aglycone and Glycosidic Flavonoids in a Caco-2 BBe1 Cell Model. ACS Omega 5:10782-10793 (2020). PubMed: 32455198
- Sunilkumar S & Ford SM Elevated glucose concentration in culture media decreases membrane trafficking of SGLT2 in LLC-PK1 cells via a cAMP/PKA-dependent pathway. Am J Physiol Cell Physiol 316:C913-C924 (2019). PubMed: 30943059