Overview

  • Product name

  • Description

    Rabbit polyclonal to SGLT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to an area surrounding amino acid 594 of Human SGLT1.

  • Positive control

    • Jurkat whole cell lysate (ab7899)

Properties

Applications

Our Abpromise guarantee covers the use of ab97682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa). A ~50 kDa cleavage fragment can also be detected in Jurkat cell lysate.

Target

  • Function

    Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.
  • Tissue specificity

    Expressed mainly in intestine and kidney.
  • Involvement in disease

    Congenital glucose/galactose malabsorption
  • Sequence similarities

    Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
  • Post-translational
    modifications

    N-glycosylation is not necessary for the cotransporter function.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • D22S675 antibody
    • High affinity sodium glucose cotransporter 1 antibody
    • High affinity sodium glucose cotransporter antibody
    • High affinity sodium-glucose cotransporter antibody
    • Human Na+/glucose cotransporter 1 antibody
    • Na(+)/glucose cotransporter 1 antibody
    • Na+/glucose cotransporter 1 antibody
    • NAGT antibody
    • SC5A1_HUMAN antibody
    • SGLT 1 antibody
    • SGLT1 antibody
    • SLC5A1 antibody
    • Sodium glucose cotransporter 1 antibody
    • Sodium/glucose cotransporter 1 antibody
    • Solute carrier family 5 (sodium/glucose cotransporter) member 1 antibody
    • Solute carrier family 5 member 1 antibody
    see all

Images

  • Anti-SGLT1 antibody (ab97682) at 4 µg/ml + Jurkat cell lysate

    Predicted band size: 73 kDa

References

ab97682 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (SDS-acrylamide 10%)
Sample
Human Cell lysate - whole cell (enteroendocrine cell)
Specification
enteroendocrine cell
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C

Abcam user community

Verified customer

Submitted Sep 17 2014

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