Overview

  • Product name
  • Description
    Rabbit polyclonal to SGLT2
  • Host species
    Rabbit
  • Specificity
    The antibody detects 74 kDa SGLT-2 from human, mouse, and rat origins. Reactivity to other species has not been determined. A ~55 kDa and a ~45 kDa band can also be detected, representing the SGLT-2 cleavage fragments.
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide near the N terminus of SGLT2 (Mouse).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Thimerosal (merthiolate)
    Constituents: 1% BSA, 50% Glycerol, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 21872923
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 73 kDa.

Target

  • Function
    Sodium-dependent glucose transporter. Has a Na(+) to glucose coupling ratio of 1:1.
    Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.
  • Involvement in disease
    Defects in SLC5A2 are the cause of renal glucosuria (GLYS1) [MIM:233100]. GLYS1 is an autosomal recessive disorder characterized by a normal fasting serum glucose concentration and persistent isolated glucosuria, with a normal glucose tolerance test.
  • Sequence similarities
    Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Low affinity sodium glucose cotransporter antibody
    • Low affinity sodium-glucose cotransporter antibody
    • Na(+) glucose cotransporter 2 antibody
    • Na(+)/glucose cotransporter 2 antibody
    • OTTHUMP00000163298 antibody
    • SC5A2_HUMAN antibody
    • SGLT 2 antibody
    • SLC5A 2 antibody
    • SLC5A2 antibody
    • Sodium glucose cotransporter 2 antibody
    • Sodium/glucose cotransporter 2 antibody
    • Solute carrier family 5 (sodium glucose cotransporter) member 2 antibody
    • Solute carrier family 5 (sodium/glucose transporter), member 2 antibody
    • Solute carrier family 5 member 2 antibody
    see all

Images

  • Immunohistochemical analysis of mouse kidney frozen section, labeling SGLT2 with ab37296. Tissue sections were fixed with acetone. Blocking was with 2% horse serum for 1 hour at room temperature. Incubation for 16 hours at 4°C with ab37296 diluted 1/100 in 2% horse serum in 1XPBS.

    See Abreview

  • All lanes : Anti-SGLT2 antibody (ab37296) at 4 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Mouse small intestine tissue lysate

    Predicted band size: 73 kDa

References

This product has been referenced in:
  • El-Daly M  et al. Hyperglycaemic impairment of PAR2-mediated vasodilation: Prevention by inhibition of aortic endothelial sodium-glucose-co-Transporter-2 and minimizing oxidative stress. Vascul Pharmacol 109:56-71 (2018). Read more (PubMed: 29908295) »
  • Xu C  et al. Canagliflozin exerts anti-inflammatory effects by inhibiting intracellular glucose metabolism and promoting autophagy in immune cells. Biochem Pharmacol 152:45-59 (2018). WB ; Mouse . Read more (PubMed: 29551587) »
See all 6 Publications for this product

Customer reviews and Q&As

1-10 of 12 Abreviews or Q&A

Application
Western blot
Sample
Guinea pig Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
50 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Christopher Trummer

Verified customer

Submitted Oct 04 2018

Application
Western blot
Sample
Rat Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
50 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Christopher Trummer

Verified customer

Submitted Oct 04 2018

Application
Western blot
Sample
Mouse Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
50 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Christopher Trummer

Verified customer

Submitted Oct 04 2018

Application
Western blot
Sample
Human Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
50 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Christopher Trummer

Verified customer

Submitted Oct 04 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Kidney)
Antigen retrieval step
None
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 05 2015

Answer


Yes, different amounts of total protein are loaded to ensure effective detection in different tissues/cells since the level of expression with respect to total protein is different. There is ˜25 ug for the Jurkat cells, ˜75-100 ug in the small intestine samples, 50 ug in the kidney samples.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (kidney)
Specification
kidney
Fixative
Acetone
Permeabilization
No
Blocking step
horse serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C

Mrs. Domenica Mccarthy

Verified customer

Submitted Apr 08 2013

Answer

DISCOUNT CODE: ****
Expiration date: dd mm yyyy

I am very pleased to hear you would like to accept our offer and test ab37296 in IHC-P. This code will give you the value of ab37296 off your next order before the expiration date. To redeem this offer, please submit an Abreview for IHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

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Answer

Thank you for contacting us.

I confirm you should suggest to your customer to apply for a testing discount if they want to use ab37296 in IHC-P or IHC-Fr.

Please note that the testing discount slightly changed very recently. It is now called "Abtrial" and after submitting their results, customers receive the value of the tested product, not just a free antibody as it used to be. For more information, please visit the Terms and Conditions page of this offer: https://www.abcam.com/abtrial.

Thank you.

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Question

Dear technical support team: This customer has purchased ab37296 (Anti-SGLT2 antibody) and has conducted the wb assay with human sample sever times. The results show no band, therefore, he wants to ask for your help to modify his experiment step, could you please offer any suggestion to help him? I also attached his data in this letter and his experiment step as follow: 1. Order details: Abcam product code: ab37296 Antibody storage conditions (temperature/reconstitution etc) -20C 2. Please describe the problem (high background, wrong band size, more bands, no band etc). No band 3. On what material are you testing the antibody in WB? ·         Species: human ·         What’s cell line or tissue : HK2 ·         Cell extract or Nuclear extract: cell extract ·         Purified protein or Recombinant protein: purified protein   3.  The lysate How much protein was loaded: 40ug What lysis buffer was used:RIPA What protease inhibitors were used: BIOMAN PIC002.1 protease inhibitor COCKTAIL What loading buffer was used: glycerol + 10%SDS+1% bromophenol blue+stacking 4X buffer+beta-mercaptoethanol Phosphatase inhibitors : no Did you heat the samples: temperature and time: 95C /5mins or 37c/10min   4.  Electrophoresis/Gel conditions/ Transfer conditions Reducing or non reducing gel: non reducing gel Reducing agent: no Gel percentage : 12.5% or 10% Transfer conditions: (Type of membrane, Protein transfer verified):  PVDF, marker 5. Blocking conditions Buffer: PBST Blocking agent: milk, BSA, serum, what percentage: milk Incubation time:1hr Incubation temperature:RT   6. Primary Antibody Species: rabbit Reacts against: human ·         At what dilution(s) have you tested this antibody:1:500~1:2000 ·         What dilution buffer was used: 5% milk in PBST ·         Incubation time: overnight ·         Incubation temperature: 4C ·         What washing steps were done: PBST*3/ 5mins   7. Secondary Antibody Species: goat Reacts against: rabbit At what dilution(s) have you tested this antibody: 1:5000~1:22000 Incubation time: 30mins Wash steps: PBST*3/ 5mins Fluorochrome or enzyme conjugate: HRP Do you know whether the problems you are experiencing come from the secondary?   8. Detection method ECl, ECl+, other detection method: ECL 9. Did you apply positive and negative controls along with the samples? Please specify.   SGLT2 protein should has high expression in HK2 cell 10. Optimization attempts ·         How many times have you tried the Western? 9 times ·         Have you eliminated the possibility that any background bands could be due to the secondary antibody? (Run a “No primary” control):  no band ·         Do you obtain the same results every time e.g. are background bands always in the same place? No band ·         What steps have you altered? Concentration of primary antibody (1:500)   Could you please help this customer to solve the problem? Thanks for your kindly help Best regards

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Answer

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

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1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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