- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-SGLT2 antibody
See all SGLT2 primary antibodies -
DescriptionMouse monoclonal to SGLT2
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Host speciesMouse
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Tested applicationsSuitable for: WB, Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment: GLFDKYLGAA TSLTVSEDPA VGNISSFCYR PRPDSYHLLR HPVTGDLPWP , corresponding to amino acids 228-278 of Human SGLT2
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General notes
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab85626 as a replacement.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
PBS, pH 7.2 -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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IsotypeIgG2a
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab58298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein. | |
| Flow Cyt | Use 1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Target
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FunctionSodium-dependent glucose transporter. Has a Na(+) to glucose coupling ratio of 1:1.
Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules. -
Involvement in diseaseDefects in SLC5A2 are the cause of renal glucosuria (GLYS1) [MIM:233100]. GLYS1 is an autosomal recessive disorder characterized by a normal fasting serum glucose concentration and persistent isolated glucosuria, with a normal glucose tolerance test.
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Sequence similaritiesBelongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
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Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 6524 Human
- Omim: 182381 Human
- SwissProt: P31639 Human
- Unigene: 709195 Human
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Alternative names
- Low affinity sodium glucose cotransporter antibody
- Low affinity sodium-glucose cotransporter antibody
- Na(+) glucose cotransporter 2 antibody
see all
Images
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Western blot - Anti-SGLT2 antibody (ab58298)Western blot against tagged recombinant protein immunogen using ab58298 SGLT2 antibody at 1ug/ml. Predicted band size of immunogen is 32 kDa -
Flow Cytometry - Anti-SGLT2 antibody (ab58298)Overlay histogram showing HEK293 cells stained with ab58298 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58298, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
References
This product has been referenced in:
- Kuang H et al. Therapeutic Effect of Sodium Glucose Co-Transporter 2 Inhibitor Dapagliflozin on Renal Cell Carcinoma. Med Sci Monit 23:3737-3745 (2017). WB ; Human . Read more (PubMed: 28763435) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"