Anti-SGLT2 antibody (ab58298)
Key features and details
- Mouse monoclonal to SGLT2
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
Overview
-
Product name
Anti-SGLT2 antibody
See all SGLT2 primary antibodies -
Description
Mouse monoclonal to SGLT2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Recombinant fragment
-
Immunogen
Recombinant fragment: GLFDKYLGAA TSLTVSEDPA VGNISSFCYR PRPDSYHLLR HPVTGDLPWP , corresponding to amino acids 228-278 of Human SGLT2
-
General notes
This product was changed from ascites to tissue culture supernatant on 12/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| WB |
Recombinant fragment
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein. |
|
| Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein. |
|
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
Target
-
Function
Sodium-dependent glucose transporter. Has a Na(+) to glucose coupling ratio of 1:1.
Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules. -
Involvement in disease
Defects in SLC5A2 are the cause of renal glucosuria (GLYS1) [MIM:233100]. GLYS1 is an autosomal recessive disorder characterized by a normal fasting serum glucose concentration and persistent isolated glucosuria, with a normal glucose tolerance test. -
Sequence similarities
Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 6524 Human
- Omim: 182381 Human
- SwissProt: P31639 Human
- Unigene: 709195 Human
-
Alternative names
- Low affinity sodium glucose cotransporter antibody
- Low affinity sodium-glucose cotransporter antibody
- Na(+) glucose cotransporter 2 antibody
see all
Images
-
Western blot - Anti-SGLT2 antibody (ab58298)
Western blot against tagged recombinant protein immunogen using ab58298 SGLT2 antibody at 1ug/ml. Predicted band size of immunogen is 32 kDa
This image was generated using the ascites version of the product.
-
Flow Cytometry - Anti-SGLT2 antibody (ab58298)Overlay histogram showing HEK293 cells stained with ab58298 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58298, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.This image was generated using the ascites version of the product.
Datasheets and documents
References (2)
ab58298 has been referenced in 2 publications.
- Dai C et al. Dapagliflozin does not directly affect human a or ß cells. Endocrinology N/A:N/A (2020). PubMed: 32428240
- Kuang H et al. Therapeutic Effect of Sodium Glucose Co-Transporter 2 Inhibitor Dapagliflozin on Renal Cell Carcinoma. Med Sci Monit 23:3737-3745 (2017). WB ; Human . PubMed: 28763435
