Overview

  • Product name

  • Description

    Rabbit polyclonal to SGPP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human SGPP1 aa 12-42 (N terminal) conjugated to keyhole limpet haemocyanin.
    Database link: Q9BX95
    (Peptide available as ab170839)

  • Positive control

    • NCI-H292 cell line lysates and Human placenta tissue.
  • General notes

    Previously labelled as sphingosine-1-phosphate phosphatase 1.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified through a protein A column, followed by peptide affinity purification.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab129253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 49 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Relevance

    SGPP1 (Spp1) regulates intracellular and extracellular sphingosine 1 phosphate(S1P)levels. It belongs to the type 2 lipid phosphate phosphatase family and is expressed in the endoplasmic reticulum (ER) membrane.
  • Database links

  • Alternative names

    • hSPPase1 antibody
    • SGPP1 antibody
    • Sphingosine-1-phosphatase 1 antibody
    • sphingosine-1-phosphate phosphatase 1 antibody
    • Spp1 antibody
    • SPPase1 antibody
    see all

Images

  • ICC/IF image of ab129253 stained JEG3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab129253 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-SGPP1 antibody (ab129253) at 1/100 dilution + NCI-H292 cell line lysates at 35 µg

    Predicted band size: 49 kDa

  • ab129253, at 1/10 to 1/50, staining SGPP1 in Human placenta tissue followed by DAB staining by immunohistochemistry [Formalin fixed and Paraffin Embedded Tissues (IHC-P)].

References

This product has been referenced in:

  • Schwiebs A  et al. Nuclear Translocation of SGPP-1 and Decrease of SGPL-1 Activity Contribute to Sphingolipid Rheostat Regulation of Inflammatory Dendritic Cells. Mediators Inflamm 2017:5187368 (2017). Read more (PubMed: 29375197) »
  • Couttas TA  et al. Loss of the neuroprotective factor Sphingosine 1-phosphate early in Alzheimer's disease pathogenesis. Acta Neuropathol Commun 2:9 (2014). WB ; Human . Read more (PubMed: 24456642) »
See all 3 Publications for this product

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There are currently no Customer reviews or Questions for ab129253.
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