Product nameAnti-SGPP1 antibody
See all SGPP1 primary antibodies
DescriptionRabbit polyclonal to SGPP1
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
- WB: RT4 cell lysate. IHC-P: Human liver tissue. ICC/IF: A431 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab262888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
|WB||Use a concentration of 0.4 µg/ml. Predicted molecular weight: 49 kDa.|
RelevanceSGPP1 (Spp1) regulates intracellular and extracellular sphingosine 1 phosphate(S1P)levels. It belongs to the type 2 lipid phosphate phosphatase family and is expressed in the endoplasmic reticulum (ER) membrane.
- hSPPase1 antibody
- SGPP1 antibody
- Sphingosine-1-phosphatase 1 antibody
PFA-fixed, Triton X-100 permeabilized human A-431 cells stained for SGPP1 (green) using ab262888 at 4 µg/ml in ICC/IF.
Paraffin-embedded human liver tissue stained for SGPP1 using ab262888 at 1/200 dilution in immunohistochemical analysis.
Anti-SGPP1 antibody (ab262888) at 0.4 µg/ml + RT4 cell lysate
Predicted band size: 49 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab262888 has not yet been referenced specifically in any publications.