RecombinantRabMAb

Recombinant Anti-SGSH/HSS antibody [EPR17312] - BSA and Azide free (ab232666)

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Immunocytochemistry/ Immunofluorescence - Anti-SGSH/HSS antibody [EPR17312] - BSA and Azide free (ab232666)
  • Immunocytochemistry/ Immunofluorescence - Anti-SGSH/HSS antibody [EPR17312] - BSA and Azide free (ab232666)
  • Anti-SGSH/HSS antibody [EPR17312] - BSA and Azide free (ab232666)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17312] to SGSH/HSS - BSA and Azide free
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-SGSH/HSS antibody [EPR17312] - BSA and Azide free
    See all SGSH/HSS primary antibodies

  • Description

    Rabbit monoclonal [EPR17312] to SGSH/HSS - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human SGSH/HSS aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P51688

  • Positive control

    • ICC/IF: MCF7 cells.

  • General notes

    ab232666 is the carrier-free version of ab200346 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab232666 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as SGSH

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab232666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
ICC/IF Use at an assay dependent concentration.

Target

  • Involvement in disease

    Defects in SGSH are the cause of mucopolysaccharidosis type 3A (MPS3A) [MIM:252900]; also known as Sanfilippo syndrome A. MPS3A is a severe form of mucopolysaccharidosis type 3, an autosomal recessive lysosomal storage disease due to impaired degradation of heparan sulfate. MPS3 is characterized by severe central nervous system degeneration, but only mild somatic disease. Onset of clinical features usually occurs between 2 and 6 years; severe neurologic degeneration occurs in most patients between 6 and 10 years of age, and death occurs typically during the second or third decade of life. MPS3A is characterized by earlier onset, rapid progression of symptoms and shorter survival.

  • Sequence similarities

    Belongs to the sulfatase family.

  • Post-translational
    modifications

    The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.

  • Cellular localization

    Lysosome.
  • Information by UniProt

  • Database links

    • Alternative names

      • Heparan sulfate sulfatase antibody
      • Heparan sulphate sulphatase antibody
      • HSS antibody
      • MPS 3A antibody
      • MPS3 A antibody
      • MPS3A antibody
      • Mucopolysaccharidosis type IIIA antibody
      • N sulfoglucosamine sulfohydrolase (sulfamidase) antibody
      • N sulfoglucosamine sulfohydrolase antibody
      • N-sulphoglucosamine sulphohydrolase antibody
      • SFMD antibody
      • SGSH antibody
      • SPHM_HUMAN antibody
      • Sulfoglucosamine sulfamidase antibody
      • Sulphamidase antibody
      • Sulphoglucosamine sulphamidase antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-SGSH/HSS antibody [EPR17312] - BSA and Azide free (ab232666)
      Immunocytochemistry/ Immunofluorescence - Anti-SGSH/HSS antibody [EPR17312] - BSA and Azide free (ab232666)

      Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling SGSH/HSS with ab200346 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HepG2 cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab200346 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200346).

    • Immunocytochemistry/ Immunofluorescence - Anti-SGSH/HSS antibody [EPR17312] - BSA and Azide free (ab232666)
      Immunocytochemistry/ Immunofluorescence - Anti-SGSH/HSS antibody [EPR17312] - BSA and Azide free (ab232666)

      Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling SGSH/HSS with ab200346 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on MCF7 cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab200346 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200346).

       

    • Anti-SGSH/HSS antibody [EPR17312] - BSA and Azide free (ab232666)
      Anti-SGSH/HSS antibody [EPR17312] - BSA and Azide free (ab232666)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab232666? Please let us know so that we can cite the reference in this datasheet.

    ab232666 has not yet been referenced specifically in any publications.

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