Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11563] to SGSM1/RUTBC2
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-SGSM1/RUTBC2 antibody [EPR11563]
See all SGSM1/RUTBC2 primary antibodies
DescriptionRabbit monoclonal [EPR11563] to SGSM1/RUTBC2
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human SGSM1/RUTBC2 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: Q2NKQ1
- Human fetal brain, Human fetal kidney and Human fetal heart tissue lysates, U87 MG cell line lysatwe, 293T cells.
This product was previously labelled as SGSM1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab171943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 129 kDa.|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Tissue specificityMainly expressed in brain, heart and testis.
Sequence similaritiesBelongs to the RUTBC family.
Contains 1 Rab-GAP TBC domain.
Contains 1 RUN domain.
Cellular localizationGolgi apparatus, trans-Golgi network.
- Information by UniProt
- KIAA1941 antibody
- RUN and TBC1 domain containing 2 antibody
- RUN and TBC1 domain containing protein 2 antibody
All lanes : Anti-SGSM1/RUTBC2 antibody [EPR11563] (ab171943) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lane 3 : Human fetal heart tissue lysate
Lane 4 : U87 MG cell line lysate
Lane 5 : 293T cell line lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 129 kDa
Flow cytometric analysis of permeabilized 293T cellslabelling SGSM1/RUTBC2 with ab171943 at 1/10 (red) or a rabbit IgG (negative) (green).
Immunofluorescent analysis of 293T cells labeling SGSM1/RUTBC2 with ab171943 at 1/100 (red). DAPI nuclear staining (blue).
ab171943 has not yet been referenced specifically in any publications.