Overview

  • Product name

  • Description

    Goat polyclonal to SGSM3
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide:

    RDHENYVACSRSHRR

    , corresponding to internal sequence amino acids 469-483 of Human SGSM3 (NP_056520.2).

  • Positive control

    • A549 lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab99422 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab99422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 3 µg/ml. Predicted molecular weight: 85 kDa.

Target

  • Function

    May play a cooperative role in NF2-mediated growth suppression of cells.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the small G protein signaling modulator family.
    Contains 1 Rab-GAP TBC domain.
    Contains 1 RUN domain.
    Contains 1 SH3 domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Bdif 1 antibody
    • Cip85 antibody
    • Cx43 interacting protein of 85 kDa antibody
    • MAP antibody
    • Merlin associated protein antibody
    • merlin binding protein antibody
    • Merlin-associated protein antibody
    • R75178 antibody
    • rab GTPase activating protein like protein antibody
    • Rab-GTPase-activating protein-like protein antibody
    • RABGAP5 antibody
    • rabGAPLP antibody
    • RUN and SH3 containing 3 antibody
    • RUN and TBC1 domain containing 3 antibody
    • RUN and TBC1 domain containing protein 3 antibody
    • RUN and TBC1 domain-containing protein 3 antibody
    • RUN and TBC1 domains-containing protein 3 antibody
    • RUSC3 antibody
    • RUTBC3 antibody
    • SGSM3 antibody
    • SGSM3_HUMAN antibody
    • Small G protein signaling modulator 3 antibody
    • Small G protein signaling modulator 3 protein antibody
    see all

Images

  • Anti-SGSM3 antibody (ab99422) at 2 µg/ml + A549 cell lysate at 35 µg

    Predicted band size: 85 kDa

References

This product has been referenced in:

  • Lin JH  et al. Small G protein signalling modulator 2 (SGSM2) is involved in oestrogen receptor-positive breast cancer metastasis through enhancement of migratory cell adhesion via interaction with E-cadherin. Cell Adh Migr 13:120-137 (2019). Read more (PubMed: 30744493) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear the customer hashad difficulty obtaining satisfactory results from this Anti-SGSM3 antibody.

The details kindly provided will provide us with vital information for our monitoring of product quality.

Indeed, I agree that the band for SGSM3 should be expected at 85 kDa (or 88 kDa for isoform 2). To be honest, I do nothave a goodexplanationwhy the customer observes a band at 70 kDa. It might have something to do with the nature of the samples: human carcinoma cells often exhibit genetic mutations that may lead to the expression of a truncated protein or a fusion protein of a different size than expected. However, this is not very well documented so far. As the protocol used by the customer seems fine to me, I am happy to offer the customer a free of charge replacement. This could either be a new vial of ab104504, or I may recommend a vial of our alternative SGSM3 antibody ab99422 to verify the results.

https://www.abcam.com/index.html?datasheet=99422 (or use the following: https://www.abcam.com/index.html?datasheet=99422).

Otherwise, I am happy to send any other primary antibody or issue a credit note in compensation.

Thank you for your cooperation. I look forward to hearing from you with details of how the customer would like to proceed.

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